375230

HGF, Mouse, Recombinant

HGF, Mouse, Recombinant Recombinant, consists of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.

• 別名: Hepatopoietin A
• UNSPSCコード: 12352203

特性

• 品質水準: 100
• アッセイ: ≥95% (as seen by a 60 kDa (α chain) and a 33 kDa (β chain) bands under reducing conditions., SDS-PAGE)
• 形状: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 不純物: <1.0 EU/μg protein Endotoxin
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

Recombinant, mouse hepatocyte growth factor (HGF) consisting of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.

生物化学的／生理学的作用

Cell Proliferation:

ED₅₀ = 10-30 ng/ml as measured in a cell proliferation assay using IMCD-3 mouse epithelial cells.



Functional ELISA

K_D<0.2 nM as measured by binding to C-Met (HGF Receptor).

警告

Toxicity: Standard Handling (A)

再構成

Reconstitute to 10 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

その他情報

Corso, S., et al. 2005. Trends Mol. Med.11, 284.
Karihaloo, A., et al. 2005. Nephron Exp. Nephrol.100, e40.
Urbanek, K., et al. 2005. Circ. Res.97, 663.
Hammond, D.E., et al. 2004. Curr. Top. Microbiol. Immunol.286, 21.
Rosario, M. and W. Birchmeier. 2004. Dev. Cell7, 3.
Gheradi, E., et al. 2003. Proc. Natl. Acad. Sci.100, 12039.
Chiu, S.J., et al. 2002. J. Biomed. Sci.9, 261.
Tanaka, Y., et al. 2002. Biochem. Biophys. Res. Commun.299, 472.
Maeshima, A., et al. 2000. Kid. Int.58, 1511.
Lesk, A.M. and W.D. Fordham. 1996. J. Mol. Biol.258, 501.
Saelman, E.U.M., et al. 1995. J. Cell Sci.108, 3531.
Mineo, R., et al. 1994. Endocrinology145, 4355.
Mizuno, K., et al. 1994. J. Biol. Chem.269, 1131.
Sasaki, M., et al. 1994. Biochem. Biophys. Res. Commun.199, 772.
Montesano, R., et al. 1991. Cell67, 901.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3