345872

Glial Derived Neurotrophic Factor, Human, Recombinant, E. coli

• 別名: rhGDNF
• UNSPSCコード: 12352202

特性

• アッセイ: ≥97% (SDS-PAGE)
• 品質水準: 100
• 形状: lyophilized
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated
• 溶解性: aqueous buffer: soluble (most); water: soluble
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

Recombinant, human glial derived neutotrophic factor expressed in E. coli. A heterogeneously glycosylated growth promoting factor that enhances neuronal survival of dopaminergic midbrain neurons. Activates receptor tyrosine kinase Ret and promotes kidney morphogenesis. Also an activator of MAP kinase. Its biological activity is destroyed by reduction of disulfide bonds.

Recombinant, human glial derived neutotrophic factor expressed in E. coli. Growth promoting factor that enhances neuronal survival of dopaminergic midbrain neurons. A heterogeneously glycosylated basic protein. Its biological activity is destroyed by the reduction of disulfide bonds.

生物化学的／生理学的作用

fully biologically active when compared to native GDNF by dose-dependent ³H-dopamine uptake assay

警告

Toxicity: Standard Handling (A)

調製ノート

The recommended stock solution is 1 µg/100µl. For more dilute samples, add a stabilizing protein such as 0.1% BSA.

再構成

Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C.

その他情報

Vega, Q.C., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10657.
Worby, C.A., et al. 1996. J. Biol. Chem. 271, 23619.
Lin, L.F., et al. 1994. J. Neurochem. 63, 758.
Lev-Fen, H., et al. 1993. Science260, 1130.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany