アッセイ
≥97% (SDS-PAGE)
品質水準
フォーム
lyophilized
メーカー／製品名
Calbiochem®
保管条件
OK to freeze
不純物
≤1 EU/μg Endotoxin (EU/μg FGF)
輸送温度
ambient
保管温度
−70°C
詳細
Recombinant, human basic fibroblast growth factor expressed in E. coli. A member of the FGF family of mitogenic peptides, comprised of seven related proteins showing 35-55% amino acid conservation within the family and 55% similarity with acidic FGF. Found in neuronal tissue (hippocampus, brain stem, peripheral ganglia, and the isocortex), pituitary, adrenal cortex, corpus luteum, and placenta. Some of the biological activities attributed to basic FGF include: mitogenic activity in vitro for cells of meso- and neuroectodermal origin (i.e. fibroblasts, endothelial cells, astrocytes, oligodendrocytes, neuroblasts, keratinocytes, bovine epithelial lens cells, osteoblasts, smooth muscle cells, and melanocytes); role in wound healing; induction of neuron differentiation, survival, and regeneration; chemoattractant effect and mitogenic activity in vitro for endothelial cells and angiogenic activity in vivo; role in tumor neovascularization. Inhibits DNA synthesis and alkaline phosphatase activity in rat osteosarcoma cell lines. Has been implicated in the development of Kaposi sarcoma in vitro. Inhibits iNOS in bovine retinal pigmented epithelial cells. Binding of FGF to heparin or cell surface heparin sulfate proteoglycans is necessary for FGF binding to its high affinity receptors. Basic and acidic FGF appear to bind to the same high affinity receptors and show a similar range of biological activities.
Recombinant, human basic fibroblast growth factor expressed in E. coli. Potent mitogen for bone cells. Inhibits DNA synthesis and alkaline phosphatase activity in rat osteosarcoma cell lines. Has been implicated in the development of Kaposi sarcoma in vitro. Inhibits inducible NOS in bovine retinal pigmented epithelial cells.
生物化学的／生理学的作用
ED₅₀ = 100-250 pg/ml as monitored in a mitogenic assay by measuring FGF basic-dependent ³H-thymidine incorporation in quiescent NR6R/3T3 fibroblasts
警告
Toxicity: Standard Handling (A)
物理的形状
Lyophilized from sterile-filtered 20 mM Tris-HCl, 1 M NaCl, 50 µg BSA/1 µg cytokine, pH 7.0.
再構成
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 6 months at -70°C.
Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing 1 mM DTT and ≥0.1% HSA or BSA.
その他情報
Goureau, O., et al. 1995. Eur. J. Biochem. 230, 1046.
Gibran, N.S., et al. 1994. J. Surg. Res. 56, 226.
Li, J.J., et al. 1993. Cancer72, 2253.
Mignatti, P., et al. 1992. J. Cell Physiol.151, 81.
Mignatti, P., et al. 1991. Proc. Natl. Acad. Sci. USA88, 11007.
Baird, A., and Klagsbrun, M. 1991. Cancer Cells3, 239.
Gospodarowicz, D. 1991. Ann. NY Acad. Sci.638, 1.
Basilico, C., et al. 1989. Ann. NY Acad. Sci.567, 95.
Burgess, W.H., and Maciag, T. 1989. Annu. Rev. Biochem.58, 575.
Rogelj, S., et al. 1989. J. Cell. Biochem.39, 13.
Rizzino, A., et al. 1988. Cancer Res.48, 4266.
Abraham, J.A., et al. 1986. EMBO J.5, 2523.
Gibran, N.S., et al. 1994. J. Surg. Res. 56, 226.
Li, J.J., et al. 1993. Cancer72, 2253.
Mignatti, P., et al. 1992. J. Cell Physiol.151, 81.
Mignatti, P., et al. 1991. Proc. Natl. Acad. Sci. USA88, 11007.
Baird, A., and Klagsbrun, M. 1991. Cancer Cells3, 239.
Gospodarowicz, D. 1991. Ann. NY Acad. Sci.638, 1.
Basilico, C., et al. 1989. Ann. NY Acad. Sci.567, 95.
Burgess, W.H., and Maciag, T. 1989. Annu. Rev. Biochem.58, 575.
Rogelj, S., et al. 1989. J. Cell. Biochem.39, 13.
Rizzino, A., et al. 1988. Cancer Res.48, 4266.
Abraham, J.A., et al. 1986. EMBO J.5, 2523.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類コード
11 - Combustible Solids
WGK
WGK 3
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
341595-25UG:
341595-UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Expression and activation of the K-fgf oncogene.
C Basilico et al.
Annals of the New York Academy of Sciences, 567, 95-103 (1989-01-01)
The fibroblast growth factor family.
A Baird et al.
Cancer cells (Cold Spring Harbor, N.Y. : 1989), 3(6), 239-243 (1991-06-01)
N S Gibran et al.
The Journal of surgical research, 56(3), 226-234 (1994-03-01)
The role of endogenous growth factors in normal wound healing is not clear. Most of the data on growth factors in healing wounds have been obtained from the application of recombinant exogenous growth factors to animal and human wounds. We
P Mignatti et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(24), 11007-11011 (1991-12-15)
Basic fibroblast growth factor (bFGF), a protein with angiogenic, mitogenic, and chemotactic properties, lacks a signal sequence and is not secreted via the classical secretory pathway. However, the growth factor is known to act extracellularly. Since no defined mechanism for
Biological activities of fibroblast growth factors.
D Gospodarowicz
Annals of the New York Academy of Sciences, 638, 1-8 (1991-01-01)
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