1.51463

Chromolith^® RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 25 mm x 4.6 mm HPLC Column

• 別名: Chromolith^® Flash RP-18 endcapped 25-4.6 HPLC column
• UNSPSCコード: 41115700
• NACRES: SB.52

特性

• product name: Chromolith^® RP-18 endcapped, L × I.D. 25 mm x 4.6 mm HPLC column
• 物質: PEEK column
• 品質水準: 100
• 認証: suitable for USP L1
• 製品種目: Chromolith^®
• 特徴: endcapped
• 包装: 1 ea of
• 標識化の程度: 18% Carbon loading
• パラメーター: 200 bar pressure; 45 °C max. temp.
• テクニック: HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (High)
• カラムL × 内径: 2.5 cm × 4.6 mm
• マトリックス: monolithic silica
• マトリックス活性基: C18 bonding phase
• ポアサイズ: 13 nm mesoporosity; 2 μm macroporosity
• operating pH range: 2-7.5
• 分離法: reversed phase
• 保管温度: no temp limit

詳細

詳細

Chromolith^® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith^® Flash RP-18 endcapped columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith^® Flash RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith^® Flash RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 25 mm length and 4.6 mm I.D. is the best choice for very rapid separations on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith^® RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.51452] with the Chromolith^® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
Or
Chromolith^® RP-18 endcapped 10-4.6 guard cartridges (3 pieces) [1.51451]
with the Chromolith^® Guard Cartridge Holder 10 mm x 4.6 mm [1.52033]
The Chromolith^® column coupler can be used to significantly increase the separation efficiency of Chromolith^® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Need more efficiency?
Chromolith^® HighResolution RP-18 endcapped 25-4.6 HPLC columns [1.52020] provide a column efficiency exceeding 140,000 plates/meter.

Learn more:
Brochure: Race through your Separations on Any System. Chromolith^® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith^® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

アプリケーション

• Rapid Determination of 30 Polyphenols in Tongmai Formula: This study utilized the Chromolith^® RP-18 endcapped column in a liquid chromatography-tandem mass spectrometry setup to analyze 30 polyphenolic compounds in a traditional Chinese medicine formula, demonstrating its application in pharmaceutical analysis and quality control of complex herbal formulations (Wang et al., 2017).


• Ultra-fast gradient LC method for omeprazole analysis using a monolithic column: The Chromolith^® RP-18 endcapped column was employed in an ultra-fast gradient liquid chromatography method for the analysis of omeprazole in enteric-coated pellets, highlighting its efficiency in pharmaceutical quality control and rapid method development (Borges et al., 2010).

アナリシスノート

Carbon content: 17.0 - 18.6 %
Pressure: ≤ 10 bar
stability testing: passes test
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 3.0 - 4.0
Tailing (USP): 0.90 - 1.55 %

法的情報

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany