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product name
Purospher® STAR RP-18 endcapped (5 µm), L × I.D. 250 mm x 2 mm LiChroCART® HPLC cartridge, manuCART™ holder required for use
物質
stainless steel column
品質水準
認証
suitable for USP L1
製品種目
Purospher®
特徴
endcapped
包装
1 ea of
標識化の程度
17% Carbon loading
パラメーター
250 bar pressure
65 °C max. temp.
テクニック
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
カラムL × 内径
25 cm × 2 mm
表面積
330 m2/g
マトリックス
fully porous particle
マトリックス活性基
C18 bonding phase
粒径
5 μm
ポアサイズ
120 Å pore size
operating pH range
1.5-10.5
分離法
reversed phase
保管温度
no temp limit
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詳細
Purospher® STAR RP-18 endcapped, 5 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B).
These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test.
Purospher® STAR RP-18 endcapped, 5 µm LiChroCART® 250-2 is a cartridge column which requires a manuCART™ cartridge column holder part number 1.51486.0001. The manuCART™ can be used to hold one cartridge column with or without direct integration of a guard column.
LiChroCART® cartridge columns mounted with a manuCART™ cartridge column holder are hand-tight only and have therefore a maximum pressure stability of 250 bar.
For higher pressure stability requirements Purospher® STAR RP-18 endcapped, 5 µm Hibar® RT 250-2 HPLC columns are available part number 1.50598.0001.
For use under UHPLC conditions (where a pressure stability up to 1000 bar is required) it is recommended to use a Purospher® STAR RP-18 endcapped, 3 µm Hibar® HR 250-2.1 UHPLC Column part number 1.50655.0001
The column dimension of 250 mm length x 2 mm I.D. is the optimal choice for HPLC separation of complex samples at lower flow rate, allowing significant solvent savings and improved sensitivity
Learn more about Purospher® STAR HPLC and UHPLC Columns
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide
These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test.
Purospher® STAR RP-18 endcapped, 5 µm LiChroCART® 250-2 is a cartridge column which requires a manuCART™ cartridge column holder part number 1.51486.0001. The manuCART™ can be used to hold one cartridge column with or without direct integration of a guard column.
LiChroCART® cartridge columns mounted with a manuCART™ cartridge column holder are hand-tight only and have therefore a maximum pressure stability of 250 bar.
For higher pressure stability requirements Purospher® STAR RP-18 endcapped, 5 µm Hibar® RT 250-2 HPLC columns are available part number 1.50598.0001.
For use under UHPLC conditions (where a pressure stability up to 1000 bar is required) it is recommended to use a Purospher® STAR RP-18 endcapped, 3 µm Hibar® HR 250-2.1 UHPLC Column part number 1.50655.0001
The column dimension of 250 mm length x 2 mm I.D. is the optimal choice for HPLC separation of complex samples at lower flow rate, allowing significant solvent savings and improved sensitivity
Learn more about Purospher® STAR HPLC and UHPLC Columns
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide
アプリケーション
Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.
特徴および利点
- Ultra-pure silica (99.999%) for excellent peak symmetry
- High separation efficiency
- Reproducible results from run-to-run and batch-to-batch
- Best all-round performance according to Tanaka
- Outstanding pH stability from pH 1.5 to 10.5
- No phase collapse when using highly aqueous mobile phases
- Good suitability for LC-MS applications
アナリシスノート
Pressure: ≤ 130 bar
Theoretical Plates (N/m) (hexylbenzene): ≥ 70000
Symmetry (hexylbenzene): 0.8 - 1.4
Capacity factor (hexylbenzene): ≥ 8.0
Theoretical Plates (N/m) (hexylbenzene): ≥ 70000
Symmetry (hexylbenzene): 0.8 - 1.4
Capacity factor (hexylbenzene): ≥ 8.0
法的情報
Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類コード
11 - Combustible Solids
試験成績書(COA)
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