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Merck

1.50037-M

Supelco

Purospher® STAR RP-18 endcapped (5 µm) HPLC Columns

L × I.D. 250 mm x 4 mm Hibar® RT HPLC Column

別名:

Purospher® STAR RP-18 endcapped (5µm) Hibar® 250-4

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About This Item

UNSPSCコード:
41115709
NACRES:
SB.52

product name

Purospher® STAR RP-18 endcapped (5 µm), L × I.D. 250 mm x 4 mm Hibar® RT HPLC column

物質

stainless steel column

品質水準

認証

suitable for USP L1

製品種目

Purospher®

特徴

endcapped

包装

1 ea of

標識化の程度

17% Carbon loading

テクニック

HPLC: suitable
LC/MS: suitable

カラムL × 内径

25 cm × 4 mm

表面積

330 m2/g

マトリックス

fully porous particle

マトリックス活性基

C18 bonding phase

粒径

5 μm

ポアサイズ

120 Å pore size

operating pH range

1.5-10.5

分離法

reversed phase

保管温度

no temp limit

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詳細

Purospher® STAR RP-18 endcapped, 5 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Thanks to these features, Purospher® STAR RP-18 endcapped, 5 µm HPLC columns allow maximum flexibility to use the best chromatographic conditions for any separation including reversed phase.Purospher® STAR RP-18 endcapped, 5 µm Hibar® RT 250-4 is a ready to use stainless steel column suitable for connection to any HPLC system.The column dimension of 250 mm length x 4 mm I.D. is the optimal choice for the high resolution HPLC separation of complex samples.The recommended guard column is Purospher® STAR RP-18 endcapped (5 µm) LiChroCART® 4-4 part number 1.50250.0001. For direct connection to a Hibar® RT columns the Precolumn holder for 4-4 LiChroCART® cartridges, part number 1.51487.0001 can be used. Item 1.50371.1003 is a Validation kit containing 3 Purospher® STAR RP-18 endcapped, 3 µm Hibar® RT 250-4 HPLC columns from 3 different base material lots.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

アプリケーション

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

特徴および利点

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

アナリシスノート

Pressure: ≤ 130 bar
Theoretical Plates (N/m) (Toluene): ≥ 80000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 1.0 - 3.0

法的情報

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

試験成績書(COA)

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