14-1054

Human recombinant histone H3.3-H4 tetramers

Human recombinant histone H3.3-H4 tetramers expressed in E Coli as fusion proteins with either an N-terminal FLAG or 6xHis epitopes & purified by Fast Protein Liquid Chromatography (FPLC).

• UNSPSCコード: 12352200
• eCl@ss: 32160405
• NACRES: NA.32

特性

• 由来生物: Escherichia coli
• リコンビナント: expressed in E. coli
• アッセイ: >98% (SDS-PAGE)
• メーカー／製品名: Upstate^®
• 濃度: 1 μg/μL
• テクニック: activity assay: suitable
• 輸送温度: dry ice

詳細

詳細

Human recombinant histone H3.3-H4 tetramers expressed in E Coli. as fusion proteins with either an N-terminal FLAG^® or 6xHis epitopes, and purified by Fast Protein Liquid Chromatography (FPLC).

Product Source: Human

The core histones, H2A, H2B, H3, and H4, form a central octameric structure that binds approximately 147 bp of DNA to form a nucleosome. The histone variant H3.3 replaces the conventional H3.1 histone in a wide range of nucleosomes in active genes, and are incorporated into chromatin independently of DNA synthesis. The structure of chromatin can be altered by post-translational modifications (PTM) occurring on the C- and N-terminal tails of core histones. Histone modifications can either provide or hinder access of key transcriptional complexes to DNA sequences and can regulate the expression of genes. These effects can be deciphered by applications such as chromatin immunoprecipiation (ChIP) which requires high quality chromatin and essential tools such as histone proteins and appropriate enzymes to assemble chromatin in vitro.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Suitable for in vitro chromatin assembly and enzyme activity assays

物理的形状

In buffer containing 20 mM Tris-Cl pH 7.9, 1 mM EDTA, 2 M NaCl, 0.5 mM PMSF and 1 mM DTT

保管および安定性

Stable at -80ºC for 1 year from date of receipt.

法的情報

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable