07-1519

Anti-phospho Grb10 (Ser503) Antibody

from rabbit, purified by affinity chromatography

• 別名: Tumor necrosis factor ligand superfamily member 4, OX40 ligand, OX40L, CD252
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: mouse
• 化学種の反応性（ホモロジーによる予測）: primate (based on 100% sequence homology), porcine (based on 100% sequence homology), human (immunogen homology)
• テクニック: inhibition assay: suitable (peptide); western blot: suitable
• NCBIアクセッション番号: NP_005302
• UniProtアクセッション番号: Q13322
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation (pSer503)
• 遺伝子情報: human ... GRB10(2887) ; mouse ... Grb10(14783) ; pig ... Grb10(100188977)

詳細

詳細

Grb10 (insulin receptor-binding protein Grb-IR) is a ubiquitous adapter protein which is expressed in both fetal and adult tissues. It is a multidomain protein that interacts with a wide array of cellular targets; the PH domain allows interaction with several subtypes of phosphoinositides; the Ras-associating domain binds a wide variety of Ras GTPases; and the SH domain facilitates binding to membrane-bound tyrosine kinase receptors which link Grb10 to different signaling pathways. Most notably, Grb10 associates with the insulin receptor at phosphotyrosine residues via its C-terminal BPS (between PH and SH) domain and inhibits the enzymatic activity of the insulin receptor. Grb10 also acts downstream of the insulin receptor pathway to inhibit activation of AKT-1. In addition, Grb10 suppresses Wnt signalling by binding to the low-density lipoprotein receptor. In fetal tissues, Grb10 may inhibit growth and placental size.

特異性

Other homologies: Rat (91% sequence homology).

This antibody recognizes Grb10 phosphorylated at (Ser503).

免疫原

Epitope: Phosphorylated (Ser503)

KLH-conjugated linear peptide corresponding to human GRB10 phosphorylated at (Ser503).

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
発生シグナル伝達

Peptide Inhibition Assay Analysis: 1 µg/mL from a representative lot detected phospho-Grb10 (Ser503) on 10 µg of embryonic fibroblast cell lysate + non-phospho-Grb10 (Ser503) peptide. However, the antibody was inhibited when incubated with a phospho-Grb10 (Ser503) peptide.

This Anti-phospho Grb10 (Ser503) Antibody is validated for use in Peptide Inhibition Assay, Western Blotting for the detection of phospho Grb10 (Ser503).

品質

Evaluated by Western Blot in mouse embryonic fibroblast cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected phospho-Grb10 (Ser503) on 10 µg of mouse embryonic fibroblast cell lysate.

ターゲットの説明

~75 kDa observed. An uncharacterized band appears at ~16 kDa in some lysates.

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Mouse embryonic fibroblast cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable