06-1074

Anti-Interleukin 21 (IL-21) Antibody

from rabbit, purified by affinity chromatography

• 別名: Za11, IL-21, IL21, Interleukin-21
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 交差性: mouse, human
• 交差性（ホモロジーによる予測）: monkey (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
• テクニック: flow cytometry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBIアクセッション番号: NP_068575
• UniProtアクセッション番号: Q9HBE4
• 輸送温度: wet ice
• 遺伝子情報: human ... IL21(59067)

詳細

詳細

Interleukin 21 (IL-21) is a cytokine that has immunoregulatory activity on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. IL-21 induces cell division/proliferation in its target cells and is expressed in activated human CD4+ T cells. IL-21 may promote the transition between innate and adaptive immunity and may play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. IL-21 expression is up-regulated in Th2 and Th17 subsets of T helper cells, as well as follicular T cells. Furthermore IL-21 is expressed in NK T cells regulating the function of these cells. IL-21 is also produced by Hodgkin′s lymphoma (HL) cancer cells and may explain a great deal of the behavior of classical Hodgkin′s lymphoma including clusters of other immune cells gathered around HL cells in cultures.

特異性

Cow, canine, equine, and porcine (93% sequence homology). Rat (86% sequence homology).

This antibody recognizes the C-terminus of IL-21.

免疫原

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human IL-21.

アプリケーション

Research Category
炎症及び免疫

Research Sub Category
サイトカイン及びサイトカイン受容体

Anti-Interleukin 21 (IL-21) Antibody is an antibody against Interleukin 21 (IL-21) for use in FC, IH(P) & WB.

Flow Cytometry Analysis: 5 µg from a previous lot detected IL-21 in human PBMC′s.

Immunohistochemistry Analysis: A 1:100 dilution from a previous lot detected IL-21 in mouse spleen tissue.

品質

Evaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected IL-21 on 10 µg of K562 cell lysate.

ターゲットの説明

A molecular weight of 18 kDa was observed; however, UniProtKB/Swiss-Prot entry Q9HBE4 (IL21_HUMAN) describes 2 isoforms produced by alternative splicing: isoform 1 at 18 kDa, isoform 2 at 17 kDa

物理的形状

Affinity purified

Purified rabbit polyclonal in PBS with 0.06% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
K562 cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10-13 - German Storage Class 10 to 13