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Unsaturated free fatty acids: a potential biomarker panel for early detection of gastric cancer.

Unsaturated free fatty acids: a potential biomarker panel for early detection of gastric cancer.

Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals (2014-10-31)

Yaping Zhang, Ling Qiu, Yanmin Wang, Chengyan He, Xuzhen Qin, Yujie Liu, Zhili Li

ABSTRACT

Changes in the levels of free fatty acids (FFAs) are closely associated with physiological status. Serum levels of C16:1, C18:3, C18:2, C18:1, C20:4, and C22:6 in 164 gastric cancer (GC) patients and 111 benign gastric disease (BGD) patients were significantly decreased compared with 252 healthy controls. Receiver operating characteristic analysis showed that the biomarker panel including C16:1, C18:3, C18:2, C20:4, and C22:6 presents a high diagnostic ability to differentiate early-stage GC patients from healthy controls plus BGD patients, with a sensitivity of 80.6% and a specificity of 72.7%.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Ammonium acetate solution, BioUltra, for molecular biology, ~5 M in H₂O

Sigma-Aldrich

Ammonium acetate, BioUltra, for molecular biology, ≥99.0%

Supelco

Methanol, analytical standard

Supelco

Ammonium acetate, LiChropur^™, eluent additive for LC-MS

Sigma-Aldrich

Acetonitrile solution, contains 0.1 % (v/v) trifluoroacetic acid, suitable for HPLC

Sigma-Aldrich

Acetonitrile solution, contains 0.05 % (v/v) trifluoroacetic acid

Supelco

Ethanol solution, certified reference material, 2000 μg/mL in methanol

Sigma-Aldrich

Acetonitrile solution, contains 0.1 % (v/v) formic acid, suitable for HPLC

Sigma-Aldrich

Ammonium acetate, 99.999% trace metals basis

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Methanol, anhydrous, 99.8%

Sigma-Aldrich

Ammonium acetate solution, for molecular biology, 7.5 M

Sigma-Aldrich

Ammonium acetate, for molecular biology, ≥98%

Sigma-Aldrich

Ammonium acetate, reagent grade, ≥98%

Supelco

Heneicosanoic acid, analytical standard

Sigma-Aldrich

Ammonium acetate, BioXtra, ≥98%

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Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)

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Ethanol, JIS special grade, 94.8-95.8%

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Ammonium acetate solution, 50 % (w/v), suitable for iron determination

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Ethanol, JIS first grade, 94.8-95.8%

Sigma-Aldrich

Ammonium acetate, JIS special grade, ≥97.0%

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Ammonium acetate, SAJ first grade, ≥97.0%

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Ammonium acetate solution, 50 % (w/v), for copper determination

Sigma-Aldrich

Methanol, JIS special grade, ≥99.8%

Sigma-Aldrich

Methanol, SAJ first grade, ≥99.5%

Sigma-Aldrich

Methanol, JIS 300, ≥99.8%, for residue analysis

Sigma-Aldrich

Methanol, suitable for HPLC

Sigma-Aldrich

Methanol, SAJ special grade

Sigma-Aldrich

Methanol, NMR reference standard

Sigma-Aldrich

Acetonitrile solution, contains 0.05 % (w/v) ammonium formate, 5 % (v/v) water, 0.1 % (v/v) formic acid, suitable for HPLC

USP

Dehydrated Alcohol, United States Pharmacopeia (USP) Reference Standard