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G005602

HPLC Analysis of Triacylglycerols (TAG) of Borago Officinalis Oil on Ascentis® Express C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)
mobile phase: [A] acetonitrile; [B] isopropyl alcohol
gradient: 0% B held for 50 min; to 70% B in 5 min; to 0% B in 1 min
flow rate: 1 mL/min
detector: MS, APCI(+); mass spectral range, 250-1200 m/z; interval, 0.5 s; scan speed, 2143 amu/s; nebulizing gas (N2) flow, 4.0 L/min; interface temperature, 400 °C; (heat block, 230 °C; desolvation line (DL) temperature, 250 °C; DL voltage, 34 V; probe voltage, +4.5 kV; Qarray voltage, 1.0 V; RF voltage, 90 V; detection gain, 1.05 kV)
injection: 2 μL
sample: Borago Officinalis, 40 mg diluted in 10 mL of hexane

suitability

application for HPLC

application(s)

flavors and fragrances

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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