ZRB1303

Anti-Anosmin Antibody, clone 2C17 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2C17, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 76.11 kDa; observed mol wt ~80 kDa
• purified by: using Protein A
• species reactivity: mouse, human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): ELISA: suitable; affinity binding assay: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_000207
• UniProt accession no.: P23352
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2C17 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Anosmin. It targets an epitope within 18 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human Anosmin.

Application

Quality Control Testing

Evaluated by Western Blotting in mouse heart tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Anosmin in mouse heart tissue lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected human recombinant Anosmin.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Anosmin in Jurkat cells.

ELISA Analysis: 300 μg/mL from a representative lot detected recombinant human Anosmin..

Affinity Binding Assay: A representative lot of this antibody bound Anosmin with a KD of 9.3 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Anosmin, clone 2C17 ZooMAb^®, Cat. No. ZRB1303, is a recombinant Rabbit monoclonal antibody that detects Anosmin and is tested for use in Affinity Binding Assay, ELISA, and Immunocytochemistry, and Western Blotting.

Target description

Anosmin-1 (UniProt: Adhesion molecule-like X-linked, Kallmann syndrome protein) is encoded by the ANOS1 (also known as ADMLX, KAL, KAL1, KALIG1) gene (Gene ID: 3730) in human. Anosmin-1 is an extracellular matrix glycoprotein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off. It is mainly a peripheral membrane protein, but it can undergo proteolytic cleavage and released into the extracellular space. It contains an N-terminal cysteine-rich region, a whey acidic protein-like four disulfide core motif (WAP), and four tandem fibronectin type III (FnIII)-like repeats, followed by a histidine-rich C terminus. Anosmin-1 is expressed in olfactory bulb, cerebellum, spinal cord, kidney, and retina during development. It is shown to be essential for axonal guidance and migration of olfactory and gonadotropin-releasing hormone (GnRH) neurons from the nasal placode to their final location in the brain. It also serves as a chemoattractant for fetal olfactory epithelial cells. It interacts with FGFR1 and induces neurite outgrowth and cytoskeletal rearrangement in embryonic GnRH olfactory neuroblasts. Mutations in ANOS1 gene resulting in loss of function are known to cause Kallmann syndrome that is characterized by anosmia and hypogonadism due to olfactory bulb dysfunction. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gonzalez-Martinez, D., et al. (2004). J. Neurosci. 24(46); 10384-10392).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb^® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable