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U2383

Sigma-Aldrich

Ubiquitin Carrier Protein H3/CDC34 human

≥95% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain

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About This Item

MDL number:
MFCD03101856
UNSPSC Code:
12352202
NACRES:
NA.56

biological source

human

Quality Level

200

recombinant

expressed in E. coli overproducing strain

Assay

≥95% (SDS-PAGE)

form

solution

mol wt

~32 kDa

UniProt accession no.

P49427
P61088

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CDC34(997) , UBE2N(7334)

Biochem/physiol Actions

Cdc34 is an essential gene for viability in yeast. Cdc34 mediates the transition from G1 to S-phase of the cell cycle by degrading the S-phase cyclin/CDK inhibitor, SIC1 in yeast and p27(KIP1) in human. Cdc34 is also known to interact with the Skp1/Cdc53/F-box (SCF) ubiquitin ligase subunits Cul1 to degrade the NF-κB inhibitor IκBα in a phosphorylation-dependent manner.
Infected cell protein 0 of herpes simplex virus 1 was shown to bind UbcH3 and induce its degradation by promoting its polyubiquination. This resulted in increased expression of an UbcH3-target gene cyclin D1 and aided in further viral infection.

Physical form

Solution in 50 mM HEPES, 200 mM NaCl, 1 mM TCEP (pH 7.5), and 10% glycerol.

Other Notes

Manufactured for Sigma by Boston Biochem. Inc.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

U2383-100UG:
U2383-VAR:
U2383-100UG-PW:
U2383-BULK:

Certificates of Analysis (COA)

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Ryan Hagglund et al.
Proceedings of the National Academy of Sciences of the United States of America, 99(2), 631-636 (2002-01-24)
Infected cell protein 0 (ICP0) of herpes simplex virus 1, a multifunctional ring finger protein, enhances the expression of genes introduced into cells by infection or transfection, interacts with numerous cellular and viral proteins, and is associated with the degradation
Evangelia Koutelou et al.
The Journal of biological chemistry, 283(7), 3846-3853 (2007-12-14)
Notch signaling constitutes an evolutionarily conserved mechanism that mediates cell-cell interactions in various developmental processes. Numerous regulatory proteins interact with the Notch receptor and its ligands and control signaling at multiple levels. Ubiquitination and endocytosis followed by endosomal sorting of

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