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TP0010

FLAG® HA Tandem Affinity Purification Kit

Synonym(s):

Anti-ddddk, Anti-dykddddk

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200

Quality Segment

200

technique(s)

affinity chromatography: suitable

storage temp.

2-8°C

General description

TAP technology is a recent development in protein purification. This technology incorporates tandem-linked affinity tags into genes of interest so that high purity fusion proteins can be isolated through two consecutive affinity purification steps. The technology is particularly useful for the isolation and identification of protein complexes by adding a tandem affinity tag to a known "bait" protein to pull down endogenous proteins that interact with the targeted protein of interest.

Application

The FLAG® HA Tandem Affinity Purification Kit is designed for the isolation of high purity FLAG-HA dual-tagged fusion proteins from complex matrix, such as cell lysates and tissue homogenates. The kit is particularly suitable for isolation of protein complexes using TAP (Tandem Affinity Purification) technology.

Sutitable for mass spectrometry analysis and western blotting.

Learn more product details in our FLAG® application portal.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Sigma-Aldrich

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Millipore

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technique(s)

affinity chromatography: suitable

technique(s)

immunoprecipitation (IP): suitable

technique(s)

immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton™ X-100, indirect ELISA: suitable, western blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein

technique(s)

-

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

400

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • F2426
    EZview™ Red ANTI-FLAG® M2 Affinity Gel, clone M2
    SDS
  • A2095
    Monoclonal Anti-HA−Agarose antibody produced in mouse, clone HA-7, purified immunoglobulin, PBS suspension
    SDS
  • R0278
    RIPA Buffer
    SDS
  • F4799
    3X FLAG® Peptide, lyophilized powder
    SDS
  • U4883
    Urea, 8 M (after reconstitution with 16 mL high purity water)
    SDS

Storage Class

10 - Combustible liquids

wgk

WGK 3

pictograms

Exclamation mark
GHS07

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

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prtr

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fsl

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ishl_indicated

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ishl_notified

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