T6074

Anti-α-Tubulin antibody, Mouse monoclonal

clone B-5-1-2, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-α-Tubulin antibody produced in mouse, alpha-Tubulin
• MDL number: MFCD00145891
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: B-5-1-2, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~50 kDa
• species reactivity: mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat
• enhanced validation: independent ( Antibodies); Learn more about Antibody Enhanced Validation
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: 0.5-1 μg/mL using cultured chicken fibroblasts (CFB); immunoprecipitation (IP): suitable; microarray: suitable; western blot: 0.25-0.5 μg/mL using total cell extract of human foreskin fibroblast cell line (FS11)
• isotype: IgG1
• UniProt accession no.: P68366
• application(s): research pathology
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TUBA4A(7277) ; mouse ... Tuba1a(22142) ; rat ... Tuba1a(64158)

Description

General description

α -tubulin, also called tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α-tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α -subunit of tubulin has molecular weight of 50,000.
Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the hybridoma B-5-1-2 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin).
Tubulin is a heterodimer that consists of α-tubulin and β-tubulin. Both subunits have a molecular weight of approx. 50 kDa and share considerable homology. In addition to α- and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven. Most of these tubulins have distinct subcellular localization and an emerging diverse set of functions. Tubulin is the major building block of microtubules. This intracellular, cylindrical, filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.
Microtubular systems contain at least three α-tubulin isoforms. Two isoforms are coded by two αt-ubulin genes, which are both transcribed and code for extremely similar proteins. The third isoform is generated by post-translational modification.

Specificity

Recognizes an epitope located at the C-terminal end of the α-tubulin isoform in a variety of organisms.

Immunogen

Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Application

Monoclonal Anti-α-Tubulin antibody produced in mouse has been used in immunoblotting.

Biochem/physiol Actions

Tubulin α 4a (TUBA4A) mutation is associated with amyotrophic lateral sclerosis (ALS).
α-Tubulin is a key regulator of cytoskeletal proteins. It mediates cellular developmental stages such as proliferation, migration, signalling and also maintains the shape of the cell. α-Tubulin controls trafficking, signaling and cellular tensegrity mediated by microtubules. The encoded protein is associated with the development and progression of cancer. α-Tubulin acetylation potentiates the metastatic property of breast cancer. Mutation in TUBA4A is associated with the development of various types of cancers, such as oral cancer, breast cancer, rectal cancer, lung cancer and prostate cancer. In addition, variation in the TUBA4A leads to sporadic amyotrophic lateral sclerosis (ALS).
Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable