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T5319

Sigma-Aldrich

Tetanolysin from Clostridium tetani

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About This Item

CAS Number:
1114581-97-3
MDL number:
MFCD03458700
UNSPSC Code:
12352200

biological source

Clostridium tetani E88

mol wt

55 kDa

application(s)

cell analysis

storage temp.

2-8°C

Gene Information

Clostridium tetani E88 ... CTC01888(1060232)

General description

Hemolytic activity of tetanolysin is determined using 2.5% rabbit red blood cells at 37 °C for 40 min.

Application

Tetanolysin has been used to analyze the formation of lytic pores in red blood cells (RBCs)1. It has also been used to permeabilize infected RBCs2.

Biochem/physiol Actions

Cholesterol-binding toxin used to permeabilize cellular membranes to enhance the entry of macromolecules into the interior of the cell. Pores induced reported to be in the range of 20-50 nm.

Preparation Note

Prepared by a modification of the method of Haque, et al.

Reconstitution

When reconstituted with 100 μl of sterile water the concentration is 1 μg/μl in 40 mM sodium phosphate buffer, pH 7.2, containing 200 mM NaCl.

Analysis Note

Single band by SDS-PAGE.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

T5319-100UG:
T5319-VAR:
T5319-BULK:
T5319-100UG-PW:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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