This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. Products that require controlled temperature storage, such as refrigeration or freezer conditions, storage temperatures are published on the product label, Certificate of Analysis, the Safety Data Sheet (SDS).
T4661
Trizma® base
≥99.9% (titration), crystalline
Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
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100 G
¥12,800
1 KG
¥60,700
5 KG
¥245,600
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100 G
¥12,800
1 KG
¥60,700
5 KG
¥245,600
About This Item
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
MDL number:
UNSPSC Code:
12352104
PubChem Substance ID:
NACRES:
NA.25
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description
aminopeptidase substrate
Quality Level
Assay
≥99.9% (titration)
form
crystalline
loss
≤5% (loss on drying, 110 °C)
color
white
pH
10.0-11.5
useful pH range
7-9
pKa (25 °C)
8.1
bp
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.)
solubility
water: soluble (400 g plus 600 mL of water)
λ
(290nm < 0.05)
in 5 mM Tris; DNA (40% (w/w))
application(s)
diagnostic assay manufacturing
SMILES string
NC(CO)(CO)CO
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key
LENZDBCJOHFCAS-UHFFFAOYSA-N
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General description
Tris is an established basimetric standard and buffer used in biochemistry and molecular biology.[1] It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc.[2] It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.[3]
Application
Tris base has been used:
- As a component of lysis buffer for cell disruption[4][5]
- In the preparation of TBE solution for PAGE (polyacrylamide gel electrophoresis)[6][5]
- In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC[7]
- In capillary electrochromatography and UV analysis of tocopherols and tocotrienols[8]
Packaging
Meets all specifications for T1503. For biotechnology, diagnostics or manufacturing use. Please inquire for commercial quantities.
Analysis Note
Not pyrogen tested. Certificates of analysis are available with lot specific information.
Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
T4661-100G:
T4661-BULK-PC:
T4661-BULK:
T4661-10KG:
T4661-VAR:
T4661-1KG:
T4661-5KG:
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Certificates of Analysis (COA)
Lot/Batch Number
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Accumulated lipids rather than the rigid cell walls impede the extraction of genetic materials for effective colony PCRs in Chlorella vulgaris.
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Genome-wide mapping of embedded ribonucleotides and other noncanonical nucleotides using emRiboSeq and EndoSeq
Ding J, et al.
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Recognition and strand displacement of DNA oligonucleotides by peptide nucleic acids (PNAs). High-performance ionexchange chromatographic analysis
Lesignoli F, et al.
Journal of Chromatography A, 922(1-2), 177-185 (2001)
Capillary electrochromatographic evaluation of vitamin active oil constituents: tocopherols and tocotrienols.
Abidi SL and Rennick KA
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