SRP8043

APRIL, Soluble mouse

recombinant, expressed in HEK 293 cells, FLAG^® tagged, >95% (SDS-PAGE)

• Synonym(s): ACRP30headless:A-proliferation-inducing ligand, ACRP30headless:APRIL, ACRP30headless:CD256, TNFSF10
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: mouse
• recombinant: expressed in HEK 293 cells
• tag: FLAG^® tagged
• Assay: >95% (SDS-PAGE)
• form: lyophilized
• mol wt: ~34 kDa by SDS-PAGE
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: <0.02 EU/μg endotoxin, tested
• color: white
• UniProt accession no.: Q9D777
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Tnfsf13(69583)

Description

General description

APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophage-mediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. APRIL is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the N-terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells.

Physical form

Lyophilized from PBS.

Reconstitution

Reconstitute with 100 μL deionized water.

Other Notes

The extracellular domain of mouse APRIL (aa 88-232) is fused at the N-terminus to mouse ACRP30headless (aa 18-110) and a FLAG^®-tag.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable