DNA Binding Protein-7 (DBP-7) is a N-terminal His-tagged recombinant protein and a member of the Sso7d family of small, abundant, non-specific DNA-binding proteins from the hyperthermophilic Archea Sulfolobus. The 7-kDa protein from Sulfolobus spp. consists of a five stranded, incomplete β-barrel capped at the opening by a C-terminal α-helix; they bind to the minor groove of a DNA duplex via the triple-stranded β-sheet. The topology of the Sulfolobus 7-kDa proteins was found to be similar to that of chromatin organization modifier (chromo) domains and eukaryotic SH3 domains, which are involved in protein–protein interactions. In vitro studies have shown that DBP-7 promotes the annealing of complementary DNA strands, induces negative supercoiling and chaperones the disassembly and renaturation of protein aggregates in an ATP hydrolysis-dependent manner.
Physical form
Sterile filtered and Lyophilized without additives.
Reconstitution
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in water to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
