SRP5344

CyclinK, GST tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): CCNK, CPR4, MGC9113
• CAS Number: 141349-86-2
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected Sf9 cells
• Assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: ~67 kDa
• NCBI accession no.: BC015935
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CCNK(8812)

Description

General description

CyclinK is a member of the cyclin family which can regulate transcription through their association with and activation of cyclin-dependent kinases (CDK). The CDK/CyclinK complex can phosphorylate the C-terminal domain (CTD) of the large subunit of RNA polymerase II. CyclinK is associated with potent CTD kinase and CDK kinase activities in vitro and coimmunoprecipitates with POLR2A. CyclinK is a new member of the transcription cyclin family which may play a dual role in regulating CDK and RNA polymerase II activities.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Preparation Note

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable