SRP4689

Prolactin from rat

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Synonym(s): Luterotropic hormone, Lutetropin, Mammotropin, PRL
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: rat
• recombinant: expressed in E. coli
• Assay: ≥95% (HPLC); ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: ~22.5 kDa
• packaging: pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: P01237
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: rat ... Prl(24683)

Description

General description

Prl (prolactin) is an anterior pituitary hormone, with a molecular weight of 23kDa. It is processed into several molecular forms such as phosphorylated, glycosylated, and lower molecular weight forms. Cleavage of the native protein results in the production of multiple N-terminal fragments of 14 to 18kDa molecular weights, known as vasoinhibins.[1]
Recombinant mouse Prolactin is a 22.5 kDa globular protein containing 198 amino acid residues.

Biochem/physiol Actions

Prl (prolactin) is processed to lower molecular weight N-terminal fragments, 14-18kDa, called vasoinhibins which have antiangiogenic effects. In endothelial cells, the 16kDa prolactin form exerts antiproliferative and proapoptotic effects which result in cell cycle arrest, caspase and NFκB (nuclear factor) activation, and regulation of Bcl-2 (B-cell lymphoma 2) protein family. In sexually mature female mice, the plasma levels of prolactin remain constant throughout estrous cycle, but show a sudden increase during proestrous cycle due to increase in circulating levels of estrogens.[1] Studies show that Prl activates T cells, B cells, natural killer (NK) cells, macrophages, neutrophils, and dendritic cells, and there is a significant level of hyperprolactinemia incidence in patients with autoimmune diseases, such as rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). Studies in rats show that Prl is involved in T cell homing to mammary glands during early lactation, and that hypoprolactemia during lactation results in modified T cell distribution with increased T cell in circulation and reduced in mammary glands.[2]

Physical form

Sterile filtered and lyophilized from 10 mM Sodium Phosphate, pH 8.0 plus 50 mM NaCl.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 1.0 mg/mL. This solution can then be diluted into other aqueous buffers.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable