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SRP3280

Sigma-Aldrich

SCD27 LIGAND human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym(s):

CD70, TNFSF7

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in CHO cells

Assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

potency

10.0-25.0 ng/mL

mol wt

25.0-35.0 kDa

packaging

pkg of 50 μg

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

P32970

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CD70(970)

General description

CD27 Ligand, a type II transmembrane protein, is expressed on activated T and B lymphocytes as well as NK cells. CD27L and its receptor (CD27) regulate the immune response by promoting T-cell expansion and differentiation, as well as NK enhancement. CD27 signaling can act as a co-stimulatory effector to sustain the survival of CD8+ T cells, primarily by inducing increased expression of the IL-2 gene.

Biochem/physiol Actions

CD27 Ligand, a type II transmembrane protein, is a member of the TNF superfamily. Full length human CD27L is a 193 amino acid protein, consisting of a 17 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 155 amino acid extracellular domain. Human soluble CD27L corresponds to the 155 amino acid extracellular domain of the full length CD27L protein. Recombinant human sCD27L contains the extracellular domain plus an N-terminal His-Tag.

Physical form

Lyophilized from 20 mM Sodium Citrate, pH 3.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SRP3280-50UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S M Lens et al.
Seminars in immunology, 10(6), 491-499 (1998-11-25)
In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors, whereas for the

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