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SRP3267

Sigma-Aldrich

NT-3 human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym(s):

HGNF, NGF-2, Nerve growth factor-2

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10 μG
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10 μG
¥62,700

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

¥62,700

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biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

20-50 ng/mL ED50

mol wt

27.2 kDa

packaging

pkg of 10 μg

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

P20783

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... NTF3(4908)

Related Categories

General description

NTF3 (neurotrophin 3) is a neurotrophic factor belonging to the NGF (nerve growth factor) family.[1] This protein is composed of 257 amino acids, with a prepro region of 138 amino acids and the mature sequence of 119 residues.[2]

Biochem/physiol Actions

NT3 functions on two structurally unrelated receptor types- the p75 neurotrophin receptor (p75NTR) and the receptor tyrosine kinase (TRK). It promotes the metastatic invasion of brain by breast cancer cells, by promoting the EMT (epithelial-mesenchymal transition) transition of breast cancer cells and suppressing the immune response in brain.[1] This protein is responsible for the indction and survival of neurite outgrowth from a subset of neural crest (NC) and placode-derived neurons.[3]
Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kDa polypeptide monomers (240 total amino acid residues).

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SRP3267-10UG:

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Certificates of Analysis (COA)

Lot/Batch Number
0911AFC62
0211AFC62

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Neurotrophin-3 modulates breast cancer cells and the microenvironment to promote the growth of breast cancer brain metastasis.
Oncogene, 32(35), 4064-4077 (2013)
P C Maisonpierre et al.
Genomics, 10(3), 558-568 (1991-07-01)
The development and maintenance of the vertebrate nervous system depends upon neuronal survival proteins known as neurotrophic factors. Nerve growth factor (NGF) remains the best characterized neurotrophic molecule. Brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) are two recently cloned neurotrophic
Neurotrophin 3 is a mitogen for cultured neural crest cells.
Kalcheim C et al
Proceedings of the National Academy of Sciences of the USA, 89(5), 1661-1665 (1992)
Cloning and expression of a cDNA encoding a novel human neurotrophic factor.
Kaisho Y et al
Febs Letters, 266(1-2), 187-191 (1990)
Ke Zhang et al.
Stem cell reviews, 10(4), 612-625 (2014-05-03)
In the past decades, mesenchymal stem cells (MSCs) as a promising cell candidate have received the most attention in the treatment of spinal cord injury (SCI). However, due to the low survival rate and low neural differentiation rate, the grafted
View All Related Papers

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