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Safety Information

SRP2119

Sigma-Aldrich

RNA Polymerase II, RPB12 subunit, GST tagged human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym(s):

ABC10-alpha, RPABC4, RPB10alpha, RPB12, RPB7.0, hRPB7.0, hsRPB10a

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

biological source

human

recombinant

expressed in E. coli

Assay

≥80% (SDS-PAGE)

form

frozen liquid

mol wt

~33 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

100 μg/mL

color

clear colorless

NCBI accession no.

NM_005034

UniProt accession no.

P53803

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... POLR2K(5440)

General description

POLR2K (RNA polymerase II subunit K) is located on human chromosome 8q22. Rpb12 is the eukaryotic subunit that is also called as ABC10α. Rpb12 is the general subunit of all three eukaryotic RNA polymerases.

Biochem/physiol Actions

RPB12 (also called RPB10 ) is shared between all three RNA polymerases and is homologous to the archaeal P subunit of RNA polymerase. Like RPB10, it is essential for yeast cell viability. RPB12 is also a zinc-binding protein with a canonical tetra-coordinating zinc motif but binds zinc in vitro much less efficiently than the RPB10 subunit. It has been shown to interact with both RPB1 and RPB2 in vitro and the human subunit can functionally substitute for its yeast counterpart.
RPB12/RPC10 (RNA polymerase II subunit K) is required for growth in Saccharomyces cerevisiae. The carboxy-terminal domain (CTD) of RNAP II plays a major role in recruiting many of these activities to the transcriptional machinery.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SRP2119-10UG:

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Certificates of Analysis (COA)

Lot/Batch Number
055108021

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N A Woychik et al.
The Journal of biological chemistry, 265(29), 17816-17819 (1990-10-15)
The Saccharomyces cerevisiae gene encoding the smallest RNA polymerase II subunit, RPB10, was isolated and sequenced. The gene for this subunit is present in single copy and maps to chromosome XV, where two other yeast RNA polymerase II subunits, RPB2
Woychik, N.A., et al.
Transcription Mechanisms and Regulation, 227-242 (1994)
F Werner et al.
Nucleic acids research, 28(21), 4299-4305 (2000-11-01)
The archaeal and eukaryotic evolutionary domains diverged from each other approximately 2 billion years ago, but many of the core components of their transcriptional and translational machineries still display a readily recognizable degree of similarity in their primary structures. The
RNA polymerase II conducts a symphony of pre-mRNA processing activities.
Howe K J.
Biochimica et Biophysica Acta, 1577(2), 308-324 (2002)
Tiling resolution array CGH and high density expression profiling of urothelial carcinomas delineate genomic amplicons and candidate target genes specific for advanced tumors.
Heidenblad M, et al.
BMC Medical Genomics, 1(1), 3-3 (2008)
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