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Safety Information

SRP2083

Sigma-Aldrich

WT-1 (-KTS) human

recombinant, expressed in insect cells, ≥60% (SDS-PAGE)

Synonym(s):

AWT1, GUD, NPHS4, WAGR, WIT-2, WT33

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77

biological source

human

recombinant

expressed in insect cells

Assay

≥60% (SDS-PAGE)

form

frozen liquid

mol wt

~55.6 kDa

packaging

pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

250 μg/mL

color

clear colorless

NCBI accession no.

NM_000378

UniProt accession no.

P19544

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... WT1(7490)

General description

Wilms tumor 1 (WT1) is encoded by the gene mapped to human chromosome 11p13. The encoded protein is expressed at high levels in nephrons, but at low levels in gonads, visceral adipose tissue, mesothelium and bone marrow cells. In addition, it is also abundantly expressed in renal podocytes in adults and in parietal epithelial cells during early days.

Biochem/physiol Actions

WT-1, the product of Wilms′ tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, inclusion or exclusion of exon 5 and the three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of WT1 protein. Such a complex post-transcriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms′ tumor. WT1 (-KTS) appears to have different binding affinity to both DNA and RNA compared to the +KTS form.
Wilms tumor 1 (WT1) is involved in various vertebrate developmental processes such as cell differentiation and proliferation, apoptosis and regulation of epithelial/mesenchymal characteristics. It serves as a tumor suppressor gene but might also possess oncogenic property. The encoded protein regulates mRNA splicing and interactions between protein. It is associated with cancers and as well as kidney function. Mutations in the gene leads to Denys-Drash syndrome, resulting in renal and genitourinary tract abnormalities. Deletion in the germline WT1 gene causes Wilms′ tumor. This gene is highly expressed in a various types of cancers.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Cartagena Act

Cartagena Act Listed

JAN Code

SRP2083-5UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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D A Haber et al.
Science (New York, N.Y.), 262(5142), 2057-2059 (1993-12-24)
A human Wilms tumor cell line (RM1) was developed to test the tumor suppressor activity of WT1, a zinc finger transcription factor that is expressed in the developing human kidney and is mutationally inactivated in a subset of Wilms tumors.
The Role of WT1 in Embryonic Development and Normal Organ Homeostasis.
Wilm B and Mu?oz-Chapuli R
Methods in Molecular Biology, 1467, 23-39 (2016)
D A Haber et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(21), 9618-9622 (1991-11-01)
The chromosome 11p13 Wilms tumor susceptibility gene WT1 appears to play a crucial role in regulating the proliferation and differentiation of nephroblasts and gonadal tissue. The WT1 gene consists of 10 exons, encoding a complex pattern of mRNA species: four
The CUG-translated WT1, not AUG-WT1, is an oncogene.
Lee K Y, et al.
Carcinogenesis, 38(12), 1228-1240 (2017)
J A Kreidberg et al.
Cell, 74(4), 679-691 (1993-08-27)
In humans, germline mutations of the WT-1 tumor suppressor gene are associated with both Wilms' tumors and urogenital malformations. To develop a model system for the molecular analysis of urogenital development, we introduced a mutation into the murine WT-1 tumor
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