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SRP0252

Sigma-Aldrich

ULK Active human

recombinant, expressed in FreeStyle™ 293-F cells, ≥40% (SDS-PAGE)

Synonym(s):

ATG1, ATG1 autophagy related 1 homolog, Serine/threonine-protein kinase ULK1, UNC-51-like kinase

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10 μG
¥119,000

¥119,000

Estimated to ship onApril 19, 2025

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10 μG
¥119,000

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

¥119,000

Estimated to ship onApril 19, 2025

biological source

human

recombinant

expressed in FreeStyle™ 293-F cells

Assay

≥40% (SDS-PAGE)

form

aqueous solution

mol wt

113 kDa

packaging

pkg of 10 μg

manufacturer/tradename

Invitrogen

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

NM_003565

UniProt accession no.

O75385

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... ULK1(8408)

General description

ULK1 (unc-51 like autophagy activating kinase 1) is a Ser/Thr protein kinase which forms a component of the ULK1 protein kinase complex. This protein complex also contains the adaptor proteins ATG (autophagy-related) 13, RB1CC1/FIP200 (RB1-inducible coiled-coil 1), and ATG101. ULK1 protein is one of the five orthologs of yeast Atg1 found in mammals.[1]

Biochem/physiol Actions

ULK1 (unc-51 like autophagy activating kinase 1) protein kinase plays a crucial role in the control of autophagy initiation. This protein is responsible for activating a cascade of signaling pathways which ultimately results in the formation of phagosomes. The phosphorylation of ULK1 by MTORC1 (mammalian target of rapamycin complex) leads to the negative regulation of autophagy.[1][2] Activation of type I interferon (IFN) receptor (IFNR) results in the activation of ULK1 protein, which, in turn, results in the transcription of gene in a IFN-stimulated response elements (ISRE) and IFNγ activation site (GAS) elements-mediated manner. It is also required for the control of the expression of key IFN-stimulated genes (ISGs).[3]

Physical form

Formulated in 2 5mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Legal Information

FreeStyle is a trademark of Invitrogen Corp.

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Certificates of Analysis (COA)

Lot/Batch Number
4009940117
40099091009
4009991009

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Deubiquitinase inhibition by WP1130 leads to ULK1 aggregation and blockade of autophagy.
Autophagy, 11(9), 1458-1470 (2015)
Computational analysis of an autophagy/translation switch based on mutual inhibition of MTORC1 and ULK1.
Szymanska P et al
PLoS ONE, 10(3), e0116550-e0116550 (2015)
Central role of ULK1 in type I interferon signaling.
Saleiro D et al
Cell Reports, 11(4), 605-617 (2015)
Pablo Sánchez-Martín et al.
Journal of cell science, 136(3) (2023-01-17)
Autophagy is a catabolic process during which cytosolic material is enwrapped in a newly formed double-membrane structure called the autophagosome, and subsequently targeted for degradation in the lytic compartment of the cell. The fusion of autophagosomes with the lytic compartment

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