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SRP0203

Sigma-Aldrich

Peroxiredoxin II Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym(s):

PRDX2, PRP, Thioredoxin-dependent peroxide reductase 1, NKEF-B, Natural killer cell-enhancing factor B, TDPX1, Thiol-specific antioxidant 1, Thioredoxin peroxidase 1

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected insect cells

Assay

≥80% (SDS-PAGE)

form

aqueous solution

specific activity

≥149 pmol/min-μg

mol wt

22.7 kDa

packaging

pkg of 100 μg

concentration

>0.02 mg/mL

technique(s)

cell based assay: suitable

NCBI accession no.

NM_005809

UniProt accession no.

P32119

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRDX2(7001)

General description

Research area: CELL SIGNALING

Peroxiredoxin 2 (PRDX2) is a typical peroxiredoxin, with 2-cysteine (Cys), a catalytic Cys51 and a resolving cysteine Cys172. Peroxiredoxins are peroxides reducing enzymes, and 2-Cys Prx subfamily contains four members in mammals, including PRDX1-4. PRDX2 is the predominant peroxidase present in mammalian erythrocytes, and its nitrated form is present in brains of patients with early Alzheimer’s disease.

Application

Peroxiredoxin II Active human has been used as a supplement in the IVM (in vitro maturation) medium for the IVM of bovine follicular oocytes.

Biochem/physiol Actions

Peroxiredoxin-2 is a ubiquitous redox-active intracellular enzyme that acts as a redox-dependent inflammatory mediator, triggering macrophages to produce and release TNF-α (tumor necrosis factor). It may be involved in peroxidase activity and resistance to overoxidation. Its levels are elevated in several human cancer cells and tissues, including colorectal cancer (CRC) and it influences diverse cellular processes involving cells survival, proliferation and apoptosis. Prdx2 plays an essential role in regulating oxidation-induced apoptosis in CRC cells and may have potential as a therapeutic target in CRC. It may also act as a possible candidate biomarker for pancreatic cancer. Prx2 is a key regulator of invasion and metastasis in melanoma.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Cartagena Act

Cartagena Act Listed

JAN Code

SRP0203-100UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Effect of peroxiredoxin II on the quality and mitochondrial activity of pre-implantation bovine embryos.
Fakruzzaman M et al
Animal Reproduction Science, 159, 172-183 (2015)
Weidong Lu et al.
Molecular and cellular biochemistry, 387(1-2), 261-270 (2013-11-16)
Peroxiredoxin 2 (Prdx2) is a member of the peroxiredoxin family, which is responsible for neutralizing reactive oxygen species. Prdx2 has been found to be elevated in several human cancer cells and tissues, including colorectal cancer (CRC), and it influences diverse
Linkage of inflammation and oxidative stress via release of glutathionylated peroxiredoxin-2, which acts as a danger signal.
Salzano S, Checconi P, Hanschmann EM, et al.
Proceedings of the National Academy of Sciences of the USA, 111(33), 12157-12162 (2014)
Lía M Randall et al.
The Journal of biological chemistry, 289(22), 15536-15543 (2014-04-11)
Peroxiredoxins (Prx) are efficient thiol-dependent peroxidases and key players in the mechanism of H2O2-induced redox signaling. Any structural change that could affect their redox state, oligomeric structure, and/or interaction with other proteins could have a significant impact on the cascade
Lucia De Franceschi et al.
Haematologica, 96(11), 1595-1604 (2011-07-14)
β-thalassemic syndromes are inherited red cell disorders characterized by severe ineffective erythropoiesis and increased levels of reactive oxygen species whose contribution to β-thalassemic anemia is only partially understood. We studied erythroid precursors from normal and β-thalassemic peripheral CD34(+) cells in
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