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SRP0147

Sigma-Aldrich

PRMT6 human

recombinant, expressed in FreeStyle 293-F cells, ≥70% (SDS-PAGE)

Synonym(s):

Arginine methyltransferase 6, HMT1 hnRNP methyltransferase-like 6, HRMT1L6

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in FreeStyle 293-F cells

Assay

≥70% (SDS-PAGE)

form

aqueous solution

mol wt

35 kDa

packaging

pkg of 20 μg

manufacturer/tradename

Invitrogen

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRMT6(55170)

General description

Human PRMT6 (GenBank Accession No. NM_018137), amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 35 kDa, expressed in FreeStyle 293-F cells.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 100 μg/ml FLAG peptide, 10% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Legal Information

FreeStyle is a trademark of Invitrogen Corp.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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