SCJ4600050

CF^™ 660C succinimidyl ester

suitable for fluorescence, ≥90% (TLC)

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• Assay: ≥90% (TLC)
• form: solid
• mol wt: ~3,112 g/mol (free acid)
• solubility: DMF: soluble; DMSO: soluble; H₂O: soluble
• ε (extinction coefficient): 200000 at 667 nm
• absorbance ratio: A_{280/667 nm} 0.08
• fluorescence: λ_ex 633, 635 or 640 nm (Flow cytometry laser line); λ_ex 633, 635 or 640 nm (Microscopy laser line); λ_ex 667 nm; λ_em 685 nm
• suitability: suitable for fluorescence
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

CF^™660C and CF^™660R are two spectrally similar fluorescent dyes that emit fluorescence at about 685 nm in the borderline spectral region between far-red and near-IR. Although their absorption maxima are at around 660 nm, both dyes can be sufficiently excited by the 633 or 635 nm laser. When combined with other CF^™ dyes of shorter wavelengths, CF^™660C or CF^™660R can serve as a useful long wavelength dye in multi-color detection applications. The two dyes are spectrally similar to Alexa Fluor^™ 660 but are far superior to the latter in performance. Like Alexa Fluor^™ 660, CF^™660C is a cyanine-based dye. However, when conjugated to protein, CF^™660C is several fold brighter and significantly more photostable than Alexa Fluor^™ 660.

Features and Benefits

1. Much brighter than Alexa Fluor^™660. 2. More photostable than Alexa Fluor^™660. 3. Highly water-soluble.

Preparation Note

Protect from light and moisture

Reconstitution

Dissolve in DMSO

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.

ALEXA FLUOR is a trademark of Life Technologies

CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable