SAB5801048

Alpaca Anti-Human IgG(Fcγ) HRP single domain Antibody

recombinant Alpaca(VHH) Nano antibody

• Synonym(s): Recombinant Alpaca single variable domain secondary Nanobody
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: alpaca
• recombinant: expressed in E. coli
• conjugate: horse radish peroxidase (HRP)
• antibody form: affinity purified immunoglobulin
• antibody product type: secondary antibodies
• clone: recombinant monoclonal
• form: lyophilized solid
• mol wt: 14 kDa
• species reactivity: human, Alpaca
• technique(s): ELISA: 1:10000-1:50000; western blot: 1:10000-1:50000
• size: 0.1 mL
• color: colorless
• isotype: VHH
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Alpaca Anti-Human IgG(Fcγ) HRP single domain Antibody is designed to specifically detect human IgG Fcγ fragment.It is a monoclonal, single domain, recombinant antibody to human IgG Fc coupled to HRP. Based on immunoelectrophoresis and/or ELISA,Alpaca Anti-Human IgG(Fcγ) HRP single domain Antibody reacts with the Fc fragment of human IgG heavy chain but not with the Fab portion of human IgG.

In mammals, antibodies are classified into five main classes or isotypes – IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgG is the most abundant antibody in normal human serum, accounting for 70-85% of the total immunoglobulin pool. Human IgG consists of four human subclasses (IgG1, IgG2, IgG3 and IgG4), and each contains a different heavy chain. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda). The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Specificity

Human IgG(Fcγ fragment specific)
Isotype: VHH domain of alpaca IgG2b/2c

Immunogen

Recombinant Alpaca single variable domain secondary nano antibody

Packaging

100 μg in Glass bottle

Physical form

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Reconstitution

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Storage and Stability

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Protect from light. Stable for 12 months at -20°C

Other Notes

Recognizes human IgG Fcγ fragment specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317,H412
• Precautionary Statements: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Hazard Classifications: Aquatic Chronic 3 - Skin Sens. 1
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable