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SAB4500233

Sigma-Aldrich

Anti-RPL35 antibody produced in rabbit

affinity isolated antibody

Synonym(s):

60S ribosomal protein L35

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 14 kDa

species reactivity

human, mouse, rat

concentration

~1 mg/mL

technique(s)

ELISA: 1:10000
western blot: 1:500-1:1000

NCBI accession no.

P42766

UniProt accession no.

P42766

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RPL35(11224)

General description

Ribosomal protein L35 (RPL35) gene codes for a ribosomal protein, a constituent of the 60S subunit. It is located on human chromosome 9q33.3. Anti-RPL35 antibody detects endogenous levels of total RPL35 protein.

Immunogen

The antiserum was produced against synthesized peptide derived from human RPL35.

Immunogen Range: 51-100

Application

Anti-RPL35 antibody produced in rabbit has been used in immunofluorescence.

Biochem/physiol Actions

Ribosomal protein L35 (RPL35) protein modulates methionine (Met)-mediated β-casein (CSN2) translational elongation and secretion. Lack of RPL35A can enhance apoptosis in hematopoietic cell lines and bone marrow cells. RPL35 gene is regarded as one of the most reliable reference genes to study gene expression in developing cacao embryos.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sayaka Yasuda et al.
Nature, 578(7794), 296-300 (2020-02-07)
The proteasome is a major proteolytic machine that regulates cellular proteostasis through selective degradation of ubiquitylated proteins1,2. A number of ubiquitin-related molecules have recently been found to be involved in the regulation of biomolecular condensates or membraneless organelles, which arise
Diamond-Blackfan Anemia
Gene (2009)
T T Pinheiro et al.
Genetics and molecular research : GMR, 10(4), 3291-3305 (2011-11-19)
Lack of continuous progress in Theobroma cacao (Malvaceae) breeding, especially associated with seed quality traits, requires more efficient selection methods based on genomic information. Reverse transcript quantitative PCR (RT-qPCR) has become the method of choice for
Xiaoyun Wu et al.
Animals : an open access journal from MDPI, 9(11) (2019-11-14)
Investigating the critical genes related to milk synthesis is essential for the improvement of the milk yield of the yak. Real-time quantitative polymerase chain reaction (RT-qPCR) is a reliable and widely used method to measure and evaluate gene expression levels.

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