SAB4300295

Anti-RELA (Ab-276) antibody produced in rabbit

affinity isolated antibody

• Synonym(s): Anti-MGC131774 antibody produced in rabbit, Anti-NFKB3 antibody produced in rabbit, Anti-p65 antibody produced in rabbit, Anti-v-rel reticuloendotheliosis viral oncogene homolog A (avian) antibody produced in rabbit
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: ~65 kDa
• species reactivity: mouse, human, rat
• concentration: 1 mg/mL
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100; indirect immunofluorescence: 1:100-1:200; western blot: 1:500-1:1000
• isotype: IgG
• immunogen sequence: (R-P-S-D-R)
• NCBI accession no.: NP_001138610.1
• UniProt accession no.: Q04206
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RELA(5970)

Description

General description

RELA (v-rel avian reticuloendotheliosis viral oncogene homolog A) codes for the important constituent of the NF-κB (nuclear factor κ-B) complex. It is located on chromosome 11q13.1. It is also called as nuclear factor κ-B subunit 3 (NFKB3).

Immunogen

Peptide sequence around aa. 274-278 (R-P-S-D-R), according to the protein RELA.

Biochem/physiol Actions

RELA (v-rel avian reticuloendotheliosis viral oncogene homolog A) participates in NFKB signaling pathway. It also controls inflammation. Alterations in the gene results in schizophrenia and their startle reactions.

Features and Benefits

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Target description

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex.

Physical form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable