SAB4200768

Anti-Human IgG1-Peroxidase antibody, Mouse monoclonal

clone 8c/6-39, purified from hybridoma cell culture

• Synonym(s): Anti-Human immunoglobulin G1
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: peroxidase conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 8c/6-39, monoclonal
• form: lyophilized
• species reactivity: human
• concentration: ~2 mg/mL
• technique(s): ELISA: 1:8,000-1:16,000 using 5 μg/ml human IgG1 for coating
• isotype: IgG2a
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Human IgGs consist of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5, and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties.Monoclonal Anti-Human IgG1 specifically recognizes Human IgG1. The antibody shows no cross-reactivity with human IgG2, IgG3, or IgG4. This clone has been established as a useful human IgG1 specificity standard by the WHO/IUIS.

Monoclonal Anti-Human IgG1 (mouse IgG2a isotype) is derived from the 8c/6-39 hybridoma (also known as HP6019), produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with the Fc fragment of a human IgG1 myeloma protein.

Immunogen

The Fc fragment of a human IgG1 myeloma protein

Application

The antibody is recommended to use in various immunological techniques, including ELISA.

Biochem/physiol Actions

The immunoglobin G (IgG) subclass may be preferentially produced in response to different antigens and pathological conditions. For instance, anti-polysaccharide responses are mainly of the IgG2 subclass while protein antigens give rise to IgG1 and IgG3 antibodies. Human IgG1 is the main antibody produced against tetanus toxoid antibodies. Elevation of IgG1 levels has been found in the cerebral spinal fluid of patients with multiple sclerosis.

Physical form

Supplied as lyophilized powder. After reconstitution with 0.1 mL of distilled water to a final antibody concentration of ∼ 2 mg/mL, the solution contains 1% BSA, 2.5% trehalose, 0.05% MIT in 0.01 M sodium phosphate buffered saline

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable