SAB4200582

Anti-Epsin1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-EPN1
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~90 kDa
• species reactivity: rat, human, mouse
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 1-2 μg/mL using extracts of SH-SY5Y cells and of mouse brain (S1 fraction).; immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded rat brain.; indirect immunofluorescence: 2-4 μg/mL using NRK cells.
• UniProt accession no.: Q9Y6I3
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EPN1(29924) ; mouse ... Epn1(13854) ; rat ... Epn1(117277)

Description

General description

There are three known mammalian epsin variants including epsin 1, 2 and 3. Epsin1 protein contains an epsin N-terminal homology (ENTH) region, a single clathrin-binding LVDLD motif and two or three tandem ubiquitin-interacting motifs (UIMs). The central region of epsin contains a clathrin interaction motif as well as repeats of a DPW motif, which binds to adaptor protein (AP)-2.

Immunogen

synthetic peptide corresponding to an internal sequence of human epsin1 isoform A, conjugated to KLH. The corresponding sequence is identical in human espin1 isoforms B and C, and in rat and mouse epsin1.

Application

Anti-Epsin1 antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

Biochem/physiol Actions

Epsin1 (also known as EPN1) is an endocytic adaptor protein that plays a central role in the clathrin-mediated internalization and degradation of ubiquinated proteins. Epsin1 recruits ubiquitinated epidermal growth factor receptor (EGFR) into clathrin-coated pits. Epsin1 is required in mitotic membrane and spindle organization. The epsin family of endocytic adaptors is found to be upregulated in cancer and epsin1 overexpression promotes cancer cell invasion.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable