SAB4200446

Anti-CD3 ζ chain antibody, Mouse monoclonal

clone ZT-10, purified from hybridoma cell culture

• Synonym(s): Anti-CD247, Anti-CD3-eta, Anti-CD3Z, Anti-CD3h, Anti-T3Z, Anti-TCRZ, Monoclonal Anti-CD3 ζ chain antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: ZT-10, monoclonal
• form: buffered aqueous solution
• species reactivity: human, mouse
• concentration: ~1.0 mg/mL
• technique(s): flow cytometry: 1.5-3.0 μg/test using Jurkat cells; indirect immunofluorescence: 2.5-5.0 μg/mL using Jurkat cells
• UniProt accession no.: P20963
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... CD247(12503)

Description

General description

Monoclonal Anti-CD3ζ chain (mouse IgG2b isotype) is derived from the hybridoma ZT-10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids 79-87 of mouse CD3 ζ chain conjugated to KLH. CD3ζ, a 16-kDa transmembrane protein, consists of a short extracellular domain and a long intracellular domain including immunoreceptor tyrosine-based activation motif (ITAM). It is a subunit of T cell receptor chains (TCR), which is expressed by T cells and natural killer (NK) cells.

The CD3ζ (CD247) gene is mapped to human chromosome 1q24.2.

Specificity

Monoclonal Anti-CD3ζ chain recognizes human and mouse CD3ζ chain.

Application

Monoclonal Anti-CD3 ζ chain antibody produced in mouse has been used in immunocytochemistry and flow cytometry.

Biochem/physiol Actions

CD3ζ regulates immunity, neuronal development and synaptic plasticity. It is vital for T cell receptor chains (TCR) complex assembly and expression. Downregulation of TCR ζ-chain expression and impairment of T-cell function linked to systemic lupus erythematosus (SLE), rheumatoid arthritis, cancer and infectious diseases. Altered expression and function of CD3ζ in T cells is associated with hematological diseases.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative

Storage and Stability

For extended storage, freeze at −20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable