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Safety Information

SAB4200151

Sigma-Aldrich

Anti-Drosha antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-ETOHI2, RANSE3L, Anti-RN3, Anti-RNASEN, Anti-RNC, Anti-RNase III, Anti-p241, Anti-ribonuclease type III, nuclear

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 130-160 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HEK-293T cells.
western blot: 2-4 μg/mL using HeLa nuclear cell lysates.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RNASEN(29102)

General description

Drosha ribonuclease III (Drosha) is encoded by the gene mapped to human chromosome 5p13.3. It is a double-stranded RNA (dsRNA)-specific RNase III enzyme. The encoded protein contains RNase III domains (RIIIDs) on the top and central domain (CED) at the bottom. In addition, it also contains unique non-canonical zinc-finger motifs.

Application

Anti-Drosha antibody produced in rabbit has been used in western blotting and immunofluorescence.

Biochem/physiol Actions

Drosha ribonuclease III (Drosha) plays a vital role in initiation of microRNA (miRNA) maturation. It catalyzes the cleavage of stem loop structure from the primary microRNA (pri-miRNA).Drosha ribonuclease III (Drosha) plays a vital role in initiation of microRNA (miRNA) maturation. It catalyzes the cleavage of stem loop structure from the primary microRNA (pri-miRNA). Mutation in the gene is associated with development of Epithelial ovarian cancer (EOC). Knockdown of Drosha can enhance the sensitivity of gastric cancer to epirubicin.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SAB4200151-200UL:
SAB4200151-BULK:
SAB4200151-25UL:
SAB4200151-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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[Knockdown of Drosha promotes chemosensitivity of epirubicin for gastric cancer MGC-803 cells].
Xu L
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 32, 1207-1211 (2016)
A novel role for GSK3? as a modulator of Drosha microprocessor activity and MicroRNA biogenesis
Fletcher CE
Nucleic Acids Research (2016)
Genome-wide Mapping of DROSHA Cleavage Sites on Primary MicroRNAs and Noncanonical Substrates
Kim B
Molecular Cell, 66, 258-269 (2017)
Peike Sheng et al.
Nucleic acids research, 46(11), 5737-5752 (2018-05-11)
MicroRNAs (miRNAs) are approximately 22 nucleotide (nt) long and play important roles in post-transcriptional regulation in both plants and animals. In animals, precursor (pre-) miRNAs are ∼70 nt hairpins produced by Drosha cleavage of long primary (pri-) miRNAs in the
Dongli Pan et al.
mBio, 10(1) (2019-02-14)
Herpes simplex virus 1 (HSV-1) switches between two infection programs, productive ("lytic") and latent infection. Some HSV-1 microRNAs (miRNAs) have been hypothesized to help control this switch, and yet little is known about regulation of their expression. Using Northern blot
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