SAB2702306

Monoclonal Anti-Histone H4K20me2 (dimethyl Lys20) antibody produced in mouse

clone GT1851, affinity isolated antibody

• Synonym(s): H4FA
• UNSPSC Code: 12352203
• NACRES: NA.41
• conjugate: unconjugated
• application: ChIP; DB; IF; IP; WB
• clone: GT1851, monoclonal
• species reactivity: human
• technique(s): ChIP: suitable; dot blot: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: 1:1,000-1:10,000

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: GT1851, monoclonal
• form: buffered aqueous solution
• mol wt: 11kDa
• species reactivity: human
• concentration: 1mg/mL
• technique(s): ChIP: suitable; dot blot: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: 1:1,000-1:10,000
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: dimethylation (Lys20)
• Gene Information: human ... HIST1H4A(8359)

Description

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4K20me2 (dimethyl Lys20).

Application

Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IP: 1:100-1:500, WB: 1:1000-1:10000, ChIP assay: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Biochem/physiol Actions

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Other Notes

Purification: Affinity purified by Protein G

Physical form

Phosphate-buffered saline, no preservative added.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable