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RAB0007

Sigma-Aldrich

Human RAGE / AGER ELISA Kit

for serum, plasma, cell culture supernatant and urine

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 2.06-1500 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... AGER(177)

General description

The Human RAGE ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human RAGE in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human RAGE

Application

For research use only. Not for use in diagnostic procedures.

Human RAGE / AGER ELISA kit has been used for the quantitative measurement of RAGE in cerebrospinal fluid.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Advanced glycosylation end-product specific receptor (AGER) or RAGE is part of the immunoglobulin superfamily of cell surface molecules. The soluble form of the protein has a function in the Wnt pathway. RAGE has a role in the development of inflammatory and cellular damage, as well as cellular toxicity. It has been associated with diabetic atherosclerosis. AGER/RAGE also modulates cell proliferation, differentiation and apoptosis.(6}

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Please refer to KIT Component information

PRTR

Please refer to KIT Component information

FSL

Please refer to KIT Component information

ISHL Indicated Name

Please refer to KIT Component information

ISHL Notified Names

Please refer to KIT Component information

Cartagena Act

Please refer to KIT Component information

JAN Code

キットコンポーネントの情報を参照してください


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Increase of Soluble RAGE in Cerebrospinal Fluid following Subarachnoid Haemorrhage
Bartosz Sokol
BioMed Research International (2017)
An inverse association between serum soluble receptor of advanced glycation end products and hyperandrogenism and potential implication in polycystic ovary syndrome patients
Yu Liao
Reproductive Biology and Endocrinology, 15, 9-9 (2017)
Association of Polymorphisms of the Receptor for Advanced Glycation Endproducts Gene with Schizophrenia in a Han Chinese Population
Jiawu Fu
Biomedical Research, 6379639-6379639 (2017)
Yin-Ching Chuang et al.
BMC pulmonary medicine, 14, 115-115 (2014-07-16)
Glutamine (GLN) has been reported to improve clinical and experimental sepsis outcomes. However, the mechanisms underlying the actions of GLN remain unclear, and may depend upon the route of GLN administration and the model of acute lung injury (ALI) used.
David G S Farmer et al.
Pulmonary pharmacology & therapeutics, 29(1), 7-14 (2014-01-15)
The calgranulin-like protein MTS1/S100A4 and the receptor for advanced glycation end-products (RAGE) have recently been implicated in mediating pulmonary arterial smooth muscle cell proliferation and vascular remodelling in experimental pulmonary arterial hypertension (PH). Here, the effects of RAGE antagonism upon

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