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Safety Information

R8507

Sigma-Aldrich

Hpa I from Haemophilus parainfluenzae

Restriction Enzyme

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About This Item

CAS Number:
81295-24-1
Enzyme Commission number:
3.1.21.4 (BRENDA, IUBMB)
MDL number:
MFCD00166572
UNSPSC Code:
12352204

grade

for molecular biology

form

buffered aqueous glycerol solution

concentration

3,000-10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

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Specificity

Recognition sequence: 5′-GTT/AAC-3′
Ligation and recutting results: After 2-10-fold Hpa I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >95% recut.
Heat inactivation: Not completely inactivated at 65 °C for 15 minutes.

Application

HpaI is a restriction endonuclease that is used to cleave DNA at the recognition site 5′-GTT/AAC-3′, generating fragments with blunt ends.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SA (B7531).

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.5 mM EDTA, 5 mM 2-mercaptoethanol, 0.01% polydocanol, 50% glycerol (v/v) at 4°C

incubation buffer

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Description
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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

JAN Code

R8507-BULK:
R8507-100UN:
R8507-500UN:
R8507-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
SLBM0183V
090M1685
090M1684
090M1604
090M1603

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L Q Gu et al.
The Journal of general physiology, 118(5), 481-494 (2001-11-07)
Noncovalent molecular adapters, such as cyclodextrins, act as binding sites for channel blockers when lodged in the lumen of the alpha-hemolysin (alphaHL) pore, thereby offering a basis for the detection of a variety of organic molecules with alphaHL as a
Nucleotide sequences at the cleavage sites of two restriction endonucleases from Hemophilus parainfluenzae.
D E Garfin et al.
Biochemical and biophysical research communications, 59(1), 108-116 (1974-07-10)
Tatiana Gianni et al.
Journal of virology, 78(22), 12268-12276 (2004-10-28)
Herpes simplex virus (HSV) enters cells by fusion with target membranes, commonly the plasma membrane. In some cells, including CHO cells expressing the nectin1 or herpesvirus entry mediator receptors, entry occurs through an endocytic route. We report the following results.
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
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