R6626

Rhodamine B

≥95% purity (HPLC), powder

• Synonym(s): Basic Violet 10, Brilliant Pink B, Rhodamine O, Tetraethylrhodamine
• Empirical Formula (Hill Notation): C₂₈H₃₁ClN₂O₃
• CAS Number: 81-88-9
• Molecular Weight: 479.01
• Colour Index Number: 45170
• Beilstein: 4091619
• EC Number: 201-383-9
• MDL number: MFCD00011931
• UNSPSC Code: 12171500
• PubChem Substance ID: 24899386
• NACRES: NA.47

Properties

• Product Name: Rhodamine B, ≥95% (HPLC)
• Quality Level: 200
• Assay: ≥95% (HPLC)
• form: powder
• technique(s): titration: suitable
• color: faint green to very dark green
• mp: 210-211 (dec.) (lit.)
• solubility: H₂O: 1 mg/mL
• density: 0.79 g/cm³
• ε (extinction coefficient): 104500-115800 at 542-554 nm in methanol
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccccc4C(O)=O)=[N+](/CC)CC)c1
• InChI: 1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H
• InChI key: PYWVYCXTNDRMGF-UHFFFAOYSA-N

Description

General description

Rhodamine B is a xanthene dye,[1] which functions as a water tracer fluorescent. It is used as a colorant in textiles and food stuffs. Rhodamine B is associated with eye, skin and respiratory tract irritation.[2] It is used as a biomarker in oral rabies vaccine.[3]

Application

Rhodamine B has been used:

• to represent fluorescence of tetra methyl rhodamine (TMR)[4]
• in the preparation of adhesive resin for confocal laser scanning microscopy[5]
• as a fluorescent dye in intrahemolymph dye dosing and retinal image acquisition[6]
• to depict brain-specific dye capture[7]
• for imaging targets[8]

Safety Information

• Pictograms: GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H302,H318,H412
• Precautionary Statements: P264 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves