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R6379

Sigma-Aldrich

Xho I from Xanthomonas holcicola

Restriction Enzyme

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About This Item

CAS Number:
81295-43-4
Enzyme Commission number:
3.1.21.4 (BRENDA, IUBMB)
MDL number:
MFCD00166586
UNSPSC Code:
12352204

grade

for molecular biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

Specificity

Recognition sequence: 5′-C/TCGAG-3′
Cutting results: a 2-10-fold Xho I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.

Application

XhoI is a resteriction endonuclease used for molecular biology methods to cut DNA molecules at the recognition sequence 5′-C/TCGAG-3′ to generate fragments with 5′-cohesive ends.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657).

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% Polydocanol (v/v) at 4 °C

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

R6379-BULK:
R6379-500UN:
R6379-2.5KU:
R6379-1KU:
R6379-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
SLBN2980V
SLBM6419V
SLBG1562V
SLBD3555V
031M1470V

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H Sesaki et al.
The Journal of cell biology, 152(6), 1123-1134 (2001-03-21)
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A new specific endonuclease present in Xanthomonas holcicola, Xanthomonas papavericola and Brevibacterium luteum.
T R Gingeras et al.
Journal of molecular biology, 118(1), 113-122 (1978-01-05)
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C Kessler et al.
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The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
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