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R4526

Sigma-Aldrich

Reference Dye for Quantitative PCR

100 ×, solution

Synonym(s):

Reference dye for qPCR, ROX

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About This Item

UNSPSC Code:
41106300
NACRES:
NA.55

form

solution

usage

sufficient for ≥600 reactions

concentration

100 ×

technique(s)

PCR: suitable

color

red

solubility

water: soluble

storage temp.

2-8°C

General description

Sigma′s Reference Dye for quantitative polymerase chain reaction (qPCR) is a proprietary dye for use with real-time PCR. It is used for the normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.

Application

Reference Dye for Quantitative PCR has been used:
  • in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR)
  • as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)
  • for analyzing the degree of cellular DNA contamination by qPCR

Other Notes

Reference Dye for Quantitative PCR is forR&D use only. Not for drug, household, or other uses.

Pictograms

Exclamation markEnvironment

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

R4526-5ML:
R4526-5ML-KC:
R4526-EW:
R4526-BULK:
R4526-.3ML-KC:
R4526-.3ML:
R4526-20RXN:
R4526PROC:
R4526-VAR:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Partial deletion of rng (RNase G)-enhanced homoethanol fermentation of xylose by the non-transgenic Escherichia coli RM10
Manow R, et al.
Journal of Industrial Microbiology & Biotechnology, 39(7), 977-985 (2012)
Ryan Manow et al.
World journal of microbiology & biotechnology, 36(4), 59-59 (2020-04-03)
An endogenous homoethanol pathway (glucose/1.2 xylose => 2 pyruvate => 2 ethanol) was previously engineered in Escherichia coli SZ410 via eliminating acid-producing pathways and anaerobic expression of the pyruvate dehydrogenase complex (aceEF-lpd operon). This ethanologenic derivative was subsequently engineered through adaptive evolution and partial
Kevin Antoine Brown et al.
Infection control and hospital epidemiology, 39(8), 917-923 (2018-08-10)
Clostridium difficile spores play an important role in transmission and can survive in the environment for several months. Optimal methods for measuring environmental C. difficile are unknown. We sought to determine whether increased sample surface area improved detection of C.
Gustav Johansson et al.
Molecular cancer therapeutics, 20(12), 2568-2576 (2021-09-24)
The majority of patients diagnosed with advanced gastrointestinal stromal tumors (GISTs) are successfully treated with a combination of surgery and tyrosine kinase inhibitors (TKIs). However, it remains challenging to monitor treatment efficacy and identify relapse early. Here, we utilized a
Sambrook, J. et al.
Molecular Cloning: A Laboratory Manual, 3rd (2000)

Articles

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

Protocols

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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