R2779
Renin human
BioUltra, recombinant, expressed in mammalian cells, ≥90 unit/μg protein, ≥98% (SDS-PAGE)
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About This Item
Application
Human renin is a member of the Peptidase A1 family. It catalyzes the hydrolysis of angiotensinogen to produce angiotensin I. It is used to study human renin-dependent hypertension as well as renin induced obesity .
Biochem/physiol Actions
Renin is a 406 amino acid precursor (45,057 Da) containing a 23 amino acid signal peptide and a 43 amino acid propeptide. Native active renin is a 340 amino acid glycoprotein containing three disulfide bonds. Renin, an aspartyl protease, cleaves angiotensinogen which results in Ang I. Ang I is converted to Ang II by the Ang-converting enzyme (ACE) . Prorenin is hydrolyzed by plasma kallekrein to produce active renin. The human renin transgene is thought to be responsible for increased appetite, obesity, and metabolic changes which are not related to angiotensin II .
Physical properties
Renin is processed as a 406 amino acid precursor (45,057 Da) containing a 23 amino acid signal peptide and a 43 amino acid propeptide. Native active renin is a 340 amino acid glycoprotein containing three disulfide bonds.
Supplied as a solution in 50 mM Tris HCl, pH 8
Unit Definition
One unit will produce one fluorescent unit from the hydrolysis of Renin Substrate I (Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys(dabcyl)-Arg) at 37 °C at pH 8.0
Preparation Note
Expressed in mammalian cells as prorenin. The prorenin is activated to renin using trypsin and re-purified.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
R2779-10UG:
R2779-VAR:
R2779-BULK:
Certificates of Analysis (COA)
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Manne Krop et al.
Hypertension (Dallas, Tex. : 1979), 61(5), 1075-1082 (2013-03-06)
Renin inhibitors like aliskiren not only block renin but also bind prorenin, thereby inducing a conformational change (like the change induced by acid) allowing its recognition in a renin-specific assay. Consequently, aliskiren can be used to measure prorenin. VTP-27999 is
Cameron J Gilbert et al.
The Canadian journal of cardiology, 29(5), 586-591 (2013-04-02)
Some evidence suggests that the direct renin inhibitor aliskiren may increase the risk of severe hyperkalemia, stroke, or acute kidney injury (AKI) when prescribed with angiotensin-converting enzyme inhibitors (ACEi's) or angiotensin-receptor blockers (ARBs). The extent to which concomitant treatment increases
Brian G Keevil
Best practice & research. Clinical endocrinology & metabolism, 27(5), 663-674 (2013-10-08)
Liquid chromatography tandem mass spectrometry (LC-MS/MS) is increasingly becoming the method of choice for steroid hormone measurements due to small sample volumes, fast analysis times and improved specificity compared to immunoassays. Achievement of demanding analytical targets for steroid analysis is
Stephen J Nicholls et al.
JAMA, 310(11), 1135-1144 (2013-09-04)
Blood pressure reduction and renin-angiotensin-aldosterone system inhibition are targets for treatment of atherosclerosis. The effect of renin inhibition on coronary disease progression has not been investigated. To determine the effects of renin inhibition with aliskiren on progression of coronary atherosclerosis.
VSL#3 ® probiotic therapy does not reduce portal pressures in patients with decompensated cirrhosis.
Saumya Jayakumar et al.
Liver international : official journal of the International Association for the Study of the Liver, 33(10), 1470-1477 (2013-08-24)
In patients with decompensated cirrhosis, bacterial translocation can contribute to splanchnic vasodilatation, decreased effective circulating volume, and portal hypertension. The primary objective of this randomized, double blind placebo controlled trial was to evaluate the effect of the probiotic VSL#3(®) on
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