Skip to Content
Merck
All Photos(1)

Documents

Safety Information

R106-05N

Sigma-Aldrich

Rat Dermal Fibroblasts: RDF, neonatal

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

rat dermis

Quality Level

100

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

morphology

Fibroblast

technique(s)

cell culture | mammalian: suitable

storage temp.

−196°C

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.


RDF from Cell Applications, Inc. have been used in allogenic prosthetic grafts, demonstrating improved epithelization, collagen organization and faster regeneration (Nomoto, 2012), in a cell sheet engineering study, where a sheet of dermal fibroblasts was transplanted into the damaged liver in allogeneic rat model to promote migration of hepatic progenitor cells and faster healing (Muraoka, 2013), and as a control in engineered cardiac tissues (Serrao, 2012).

Cell Line Origin

Skin

Application

Injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response.

Components

Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO

Quality

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

Preparation Note

  • 1st passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO
  • Can be cultured at least 8 population doublings

Subculture Routine

Please refer to the RDF Culture Protocol.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

R106-05N:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service