PROTMEM

ProteoPrep^® Membrane Extraction Kit

• NACRES: NA.32
• UNSPSC Code: 12352200
• Storage temp.: 2-8°C

Properties

• Quality Segment: 200
• input: general use sample(s)
• storage temp.: 2-8°C

Description

General description

ProteoPrep^® Membrane Extraction Kit reagents utilize a powerful detergents for higher loading and resolution of proteins in 2D gels. Protein Extraction Reagent Types 3 and 4 are optimized for proteomic studies. Extraction reagents 3 and 4 are ideal for protein extraction prior to isoelectric focusing and 2D electrophoresis.[1] This kit also includes reagents for the reduction and alkylation of disulfide bonds. The ProteoPrep^® Membrane Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.

Application

ProteoPrep^® Membrane Extraction Kit is designed to prepare highly enriched membrane protein solutions from many types of cells. The final protein solution are suitable for 2D gel electrophoresis.[2]

Features and Benefits

• Innovative detergent preparations - Improved solubility allows for higher protein loads and greater visibility of low abundance proteins in 2D gels.
• Two pre-mixed solubilization solutions - Removes interfereing non-membrane proteins prior to extractions, resulting in uncluttered 2D arrays.
• Pre-measured reducing & alkylating reagents - Easy-to-use reagents provide improved IEF resolution.

Legal Information

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

• T7567

  Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5

  SDS
• C0356

  Protein Extraction Reagent Type 4 23 mL

  SDS
• A3221

  Iodoacetamide, Single use vial of 56 mg 5 x 56

  SDS

Safety Information

• pictograms: GHS06,GHS08,GHS05,GHS09
• signalword: Danger
• hcodes: H301,H314,H317,H334,H335,H351,H360Df,H411
• pcodes: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• flash_point_f: 186.8 °F - closed cup
• flash_point_c: 86 °C - closed cup
• wgk: WGK 3