PD1

PeroxiDetect^™ Kit

• Synonym(s): Peroxidase assay kit
• UNSPSC Code: 41116158
• NACRES: NA.32

Properties

• Quality Level: 200
• storage temp.: 2-8°C

Description

General description

The PeroxiDetect Kit For the Determination of Aqueous and Lipid Hydroperoxides was developed for the measurement of peroxides in biological systems, which is an important factor in determining the degree of free radicals present in specific tissues.

Application

For the determination of aqueous peroxides and lipid hydroperoxides. Peroxides serve as a source for hydroxyl or peroxyl reactive radicals. These radicals can interact with DNA, proteins or lipid components of cells, causing cell damage that could lead to cell death. The measurement of peroxides in biological systems is an important factor in determining the degree of free radicals present in specific tissues. Lipid peroxidation has been proposed to contribute to various pathophysiological cell and tissue abnormalities. For example, increased levels of lipid peroxidation products in red blood cells were found to correlate well with the onset of diabetes mellitus in pregnant women. Cholesterol hydroperoxides accumulate in patients having excessive blood alcohol. Measurement of hydrogen peroxide in tissues has been used to study several aspects of free radical damage such as skin aging as induced by UV light and the effect of H₂O₂ as an inducer of elevated tyrosinase levels in melanoma cells. H₂O₂ has been shown to be a potent mitogen for growth-arrested cultured human aortic smooth muscle cells. The PeroxiDetect kit is capable of detection of H₂O₂ in aqueous solutions in the range of 1-7 nanomoles per reaction volume or of lipid hydroperoxides in organic solvents in the range of 1-16 nanomoles per reaction volume. This kit is based on the fact that peroxides will convert Fe²⁺ ion to Fe³⁺ ion at acidic pH. The Fe³⁺ ion will form a colored adduct with xylenol orange which is observed at 560 nm.

PeroxiDetect^™ Kit is suitable for use:

• in the quantification of hydroperoxides in synaptosomal preparation
• in measuring lipid peroxidation in melanoma cells
• to quantify peroxides in the cerebral cortex
• intestinal lumen sample

Peroxides will convert Fe²⁺ to Fe³⁺ ions under acidic conditions. Fe³⁺ ions will then form a colored adduct with xylenol orange, which is observed at 560 nm.

Components

Kit contains reagents for 100 tests of aqueous peroxide and 100 tests of organic peroxide.

Legal Information

PeroxiDetect is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

• tert-Butyl hydroperoxide 1 mL
• Hydrogen peroxide 1 mL

Safety Information

• Pictograms: GHS02,GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H242,H290,H302,H311,H314,H317,H330,H335,H341,H410
• Precautionary Statements: P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P370 + P378
• Hazard Classifications: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Org. Perox. C - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 5.2 - Organic peroxides and self-reacting hazardous materials
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable