Skip to Content
Merck
All Photos(1)

Documents

Safety Information

P9614

Sigma-Aldrich

Lambda protein phosphatase

liquid, Bacteriophage Lambda, recombinant, expressed in E. coli

Synonym(s):

Lambda-PPase

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
401941-75-1
MDL number:
MFCD00243301
UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

200

form

liquid

mol wt

25 kDa

concentration

≥400000 units/mL

shipped in

dry ice

storage temp.

−70°C

General description

Lambda protein phosphatase is a recombinant protein expressed in Escherichia coli. It requires Mn2+ or Ni2+ as activators.

Biochem/physiol Actions

Lambda protein phosphatase has been used to dephosphorylate HeLa cell extracts.
Can be used to release phosphate groups from serine, threonine or tyrosine residues in proteins. Also active on phosphorylated histidine residues.

Unit Definition

One unit will hydrolyze 1 nanomole of p-nitrophenyl phosphate per min at pH 7.5 at 30 deg C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

P9614-20KU:
P9614-BULK:
P9614-20KU-PW:
P9614-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Proliferating cell nuclear antigen-dependent rapid recruitment of Cdt1 and CRL4Cdt2 at DNA-damaged sites after UV irradiation in HeLa cells.
Ishii T
The Journal of Biological Chemistry, 285(53), 41993-42000 (2010)
Expression, purification, crystallization, and biochemical characterization of a recombinant protein phosphatase.
Zhuo S
The Journal of Biological Chemistry, 268(24), 17754-17761 (1993)
P T Cohen et al.
The Biochemical journal, 260(3), 931-934 (1989-06-15)
Infection of Escherichia coli with phage lambda gt10 resulted in the appearance of a protein phosphatase with activity towards 32P-labelled casein. Activity reached a maximum near the point of cell lysis and declined thereafter. The phosphatase was stimulated 30-fold by
Xiaoyan Yu et al.
The Journal of comparative neurology, 529(3), 481-500 (2020-05-26)
Afferent activity dynamically regulates neuronal properties and connectivity in the central nervous system. The Fragile X mental retardation protein (FMRP) is an RNA-binding protein that regulates cellular and synaptic properties in an activity-dependent manner. Whether and how FMRP level and
Kyoko Chiba et al.
Molecular biology of the cell, 28(26), 3857-3869 (2017-11-03)
In neurons, amyloid β-protein precursor (APP) is transported by binding to kinesin-1, mediated by JNK-interacting protein 1b (JIP1b), which generates the enhanced fast velocity (EFV) and efficient high frequency (EHF) of APP anterograde transport. Previously, we showed that EFV requires
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service