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P9290

Sigma-Aldrich

Proteinase K–Agarose from Tritirachium album

lyophilized powder

Synonym(s):

Proteinase K-Agarose

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10 UNITS
¥45,360
25 UNITS
¥90,400

¥45,360

List Price¥56,700Save 20%

Estimated to ship onFebruary 25, 2025

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10 UNITS
¥45,360
25 UNITS
¥90,400

About This Item

MDL number:
MFCD00132131
UNSPSC Code:
41106500
NACRES:
NA.56

¥45,360

List Price¥56,700Save 20%

Estimated to ship onFebruary 25, 2025

biological source

microbial (T.album
T. ALBUM)

Quality Level

200

form

lyophilized powder

extent of labeling

30-140 units per g solid

technique(s)

DNA extraction: suitable
RNA extraction: suitable
protein extraction: suitable

swelling

1 g swells to ~5 mL

storage temp.

−20°C

Application

Proteinase K-agarose is used in protein and nucleic acid isolation, modifying enzymes and immobilized enzymes. Proteinase K-agarose has been used to study Klebsiella pneumonia as well as Echinococcus multilocularis infection in domesticated dogs.

Biochem/physiol Actions

The application of proteinase K has been extended by immobilizing it on an insoluble matrix such as agarose. The agarose is quite inert in most systems and allows high activities per unit weight. This reactive resin allows the enzyme reaction to be quickly catalyzed by momentary contact with the substrate in a suitable medium. Reactions can be carried out in a batch slurry or even through a small column. In either case, the insoluble enzyme does not contaminate the reaction mix, which can be readily filtered out of the batch slurry or remain behind in the column.

Features and Benefits

• Recylable Proteinase K on matrix support.
• Spin column or batch processing possible.

Unit Definition

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Physical form

Lyophilized powder stabilized with lactose

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

P9290-BULK:
P9290-VAR:
P9290-10UN:
P9290-5UN:
P9290-25UN:

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Certificates of Analysis (COA)

Lot/Batch Number
0000371563
0000365517
0000371563
0000316980
0000316982

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