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P8986

Sigma-Aldrich

Peroxiredoxin 1 human

≥85% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Synonym(s):

NKEFA

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About This Item

MDL number:
MFCD03457969
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

Quality Level

200

recombinant

expressed in E. coli

Assay

≥85% (SDS-PAGE)

form

lyophilized powder

UniProt accession no.

Q06830

application(s)

cell analysis

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PRDX1(5052)

General description

Peroxiredoxin 1, an active enzyme, has two cysteine residues which correspond to Cys 47 and Cys 170 of yeast TPx (thioredoxin peroxidase).
Two cysteine residues correspond to Cys 47 and Cys 170 of yeast TPx (thioredoxin peroxidase).

Specificity

This product has been shown to catalyze NADPH oxidation in the thioredoxin-thioredoxin reductase system.

Application

Peroxiredoxin 1 has been used as a standard in enzyme assay for Clostridium difficile recombinant spore coat protein, cotE (with peroxiredoxin and chitinase activity).

Biochem/physiol Actions

Peroxiredoxin 1 has a role in cellular peroxide scavenging and it modulates various signaling pathways. It is over-expressed in many cancer cells. It regulates cell growth and signaling by interacting with proteins like c-jun-N-terminal kinase and c-myc.
Peroxiredoxins are a novel defined family of peroxidases of approximately 25 kDa that reduce H2O2 and alkyl hydroperoxides and use mainly the thioredoxin (Trx) system (Trx, thioredoxin reductase and NADPH) as electron donor. The peroxiredoxin family includes more than 30 proteins from organisms of all kingdoms. Peroxiredoxin I belongs to the Type I Peroxiredoxin family. This family consists of human natural killer cell enhancing factor (NKEFA), human proliferation associated gene (PAG), mouse macrophage stress induced protein (MSP23), mouse osteoblast specific factor (OSF-3), and rat heme-binding protein (HBP23). These proteins share about 95% homology.

Physical form

Lyophilized powder containing HEPES buffer salts and trehalose as stabilizer.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

P8986-VAR:
P8986-250UG:
P8986-250UG-PW:
P8986-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
056M4079V
11131206
056M4079
051M4040
11100513

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H Z Chae et al.
The Journal of biological chemistry, 269(44), 27670-27678 (1994-11-04)
A 25-kDa antioxidant enzyme that provides protection against oxidation systems capable of generating reactive oxygen and sulfur species has previously been identified. The nature of the oxidant eliminated by, and the physiological source of reducing equivalents for, this enzyme, however
Overexpression of Prdx1 in hilar cholangiocarcinoma: a predictor for recurrence and prognosis.
Zhou J
International Journal of Clinical and Experimental Pathology, 8(9), 9863-9874 (2015)
Functional characterization of Clostridium difficile spore coat proteins.
Permpoonpattana P
Journal of Bacteriology, 195(7, 1492-1503 (2013)
A family of novel peroxidases, peroxiredoxins.
S G Rhee et al.
BioFactors (Oxford, England), 10(2-3), 207-209 (1999-12-28)
Chia-Hsiung Liu et al.
Journal of translational medicine, 14(1), 114-114 (2016-05-05)
Extracellular peroxiredoxin 1 (Prdx1) has been implicated to play a pivotal role in regulating inflammation; however, its function in tissue hypoxia-induced inflammation, such as severe cardiogenic shock patients, has not yet been defined. Thus, the objective of this study was
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