P6486

EZview^™ Red Protein A Affinity Gel

• Synonym(s): EZview Affinity Gel, Protein A Affinity Gel
• MDL number: MFCD03456742
• UNSPSC Code: 12352202
• NACRES: NA.56

Properties

• form: liquid
• analyte chemical class(es): proteins (Immunoglobulins of various mammalian species)
• technique(s): affinity chromatography: suitable; immunoprecipitation (IP): suitable
• capacity: ~4 mg binding capacity (rabbit IgG)
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

EZview^™ Red Protein A Affinity Gel is a highly visible, red colored Protein A affinity gel. The affinity resin contains Staphylococcus aureus Protein A covalently attached to crosslinked 4% agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue. The immune complexes, bound to the EZview^™ Red Protein A Affinity Gel, are recovered by centrifugation. Red dye enhances visibility for more efficient results.

Application

EZview^™ Red Protein A Affinity Gel has been used for purification of proteins in the preparation of immunoprecipitation[1][2] and chromatin immunoprecipitation (ChIP)[3] assays.

When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview^™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Features and Benefits

• Increased visibility - Red color reduces risk of incidental aspiration
• Improved recovery of target protein by reduced accidental loss
• Higher reproducibility - More consistent yields

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

50% slurry in PBS with 50%Glycerol and 0.0015%

Storage and Stability

EZview^™ Red Protein A Affinity Gel should be stored in 50% glycerol at -20 °C. This product is stable for at least one year when stored as indicated. Do not freeze in the absence of glycerol.

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.

Safety Information

• Pictograms: GHS07,GHS09
• Signal Word: Warning
• Hazard Statements: H315,H317,H319,H411
• Precautionary Statements: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves